Abstract: This paper reports the results of a quantitative study about the free amino acids(FAA) within hemolymph of Anopheles sinensis adult females fed on blood meal infected with Plasmodium vivax and those fed on uninfected blood meal.The hemolymph was collected by centrifuging incised mosquitoes, a method described by Mack and Vanderberg ( 1978 ) with a little modification. Quantitative determination was made with high performance liquid chromatography(LC-6A). Measurements were taken right after mosquitoes' emergence and on the 5th, 12th and 18th days after the blood meal. Twenty amino acids were detected in mosquito hemolymph and the regular changes of amounts of them observed.1. The total FAA of mosquitoes who had an uninfected blood meal (MN) rose 37% on the 5th day after the blood meal, and remained relatively constant, and then dropped slightly around day 12, and reincreased again thereafter. On the 18th day, it got up to 2.7 times.2. The total FAA of the mosquitoes who had an infected blood meal (MI) reduced 9.3% after the blood meal and kept reducing for about 12 days, and then, on the 18th day, rose about 50%. The general level was much lower than that of total FAA of MN.3. The level changing behaviour of individual amino acid of MI was different from that of MN significantly.①His. Asn. Lys. and Glu. kept reducing during the 18 days after the blood meal.②There was a significant reduction in Ala. Val. Leu.on day 5 and day 12 after the blood meal and Gin. on day 12 and day 18.③Level of Asp. dropped to zero on day 5 and 12 and reappeared on the 18th day.④Met. kept on reducing and got zero before the 12th day.⑤Gln. increased in the first 5 days and dropped thereafter.

摘要： 初次仿用Mack和Vanderberg(1978)剖切、离心法,稍加改良,提取蚊血淋巴,用高效液相色谱仪(LC-6A),检测非感染和感染间日疟原虫血餐中华按蚊生命期(刚羽化后,第1次血餐后5、12及18天,血淋巴中游离氨基酸20种,经T检验(P<0.05,相差明显;P<0.01,相差显著)和分析的结果表明:①非感染血餐蚊血淋巴游离氨基酸总量,在初次血餐后一直维持在增加的水平,但血餐后12天增加的量此笫5天稍低,18天又剧增,达2.7倍;②感染与非感染间日疟原虫血餐蚊血淋巴游离氨基酸比较:感染蚊游离氨基酸总量在血餐后5天比刚羽化时减少9.3%,12天持续减少,18天又增加,达50%,但均较非感染蚊的为低;组氨酸(His)、天门冬酰胺(Asm)、赖氨酸(Lys)、谷氨酸(Glu)在感染蚊整个生命期中,均较非感染蚊明显或显著减少;鸟氨酸(Orn)于感染血餐后5及12天消失;18天又复出现且量有增加:蛋氨酸(Met)量一直减少,且于血餐后12天消失;仅谷氨酰胺(Gln)于感染血餐后5天较非感染蚊的量明显增加。

关键词: 间日疟原虫, 中华按蚊, 血淋巴, 氨基酸