Abstract: Objective To investigate the current status of resistance of Musca domestica to four commonly used insecticides in Bijie and Fuquan of Guizhou province, China, and to provide a basis for scientific prevention and control of M. domestica and rational selection of insecticides. Methods In August 2018, the net trap method was used to capture adult M. domestica at breeding places in Bijie and Fuquan, and the flies were brought back to insectary for identification and were fed to obtain the F1 generation. The micro-drip method was used to determine the resistance of adult female M. domestica, aged 3-5 days after eclosion, to four commonly used insecticides. SPSS 24.0 software was used to perform a statistical analysis and calculate median lethal dose (LD₅₀). Results The LD₅₀ values of M. domestica in Bijie against deltamethrin, beta-cypermethrin, permethrin, and DDVP were 0.141, 0.488, 0.288, and 0.627 μg/♀, respectively, with resistance ratios of 20.14, 28.71, 57.60, and 209.00 folds, respectively. The LD₅₀ values of M. domestica in Fuquan against the above four insecticides were 0.180, 0.450, 0.464, and 0.808 μg/♀, respectively, with resistance ratios of 25.71, 26.47, 92.80, and 269.33 folds, respectively. Conclusion M. domestica in Bijie and Fuquan has developed resistance to the four commonly used insecticides, with a consistent level of resistance. M. domestica in both cities has the highest level of resistance to DDVP and high levels of resistance to the other three pyrethroid insecticides. Chemical insecticides should be effectively selected according to the insecticide resistance results so as to improve the efficacy and efficiency of the prevention and control of flies.

Key words: Musca domestica, Insecticide, Insecticide resistance, Micro-drip method

摘要： 目的 了解贵州省毕节和福泉市家蝇对4种常用杀虫剂的抗药性现状，为家蝇的科学防控及杀虫剂的合理选用提供依据。方法 2018年8月在贵州省毕节和福泉市蝇类孳生场所以网捕法采集家蝇成虫，带回实验室鉴定并饲养至F1代，采用微量点滴法测定羽化后3~5日龄的健康雌性成蝇对4种常用杀虫剂的抗药性，利用SPSS 24.0软件进行统计学分析并计算其半数致死量（LD₅₀）。结果 毕节市家蝇对溴氰菊酯、高效氯氰菊酯、氯菊酯及敌敌畏（DDVP）的LD₅₀值分别为0.141、0.488、0.288和0.627μg/♀，抗性倍数分别为20.14、28.71、57.60和209.00倍；福泉市家蝇对溴氰菊酯、高效氯氰菊酯、氯菊酯及DDVP的LD₅₀值分别为0.180、0.450、0.464和0.808 μg/♀，抗性倍数分别为25.71、26.47、92.80和269.33倍。结论 毕节和福泉市家蝇对4种常用杀虫剂均已产生抗药性，且抗性级别一致。两地家蝇对DDVP均为极高抗水平，对3种拟除虫菊酯类杀虫剂均为高抗水平，建议该地根据抗药性监测结果，选用敏感化学杀虫剂，提高蝇类防制效果和效率。

关键词: 家蝇, 杀虫剂, 抗药性, 点滴法