Chines Journal of Vector Biology and Control ›› 2019, Vol. 30 ›› Issue (1): 7-11.DOI: 10.11853/j.issn.1003.8280.2019.01.002

Previous Articles     Next Articles

Genotypes of knockdown resistance gene and their distribution in Aedes albopictus in Haikou, China, in 2018

ZHAO Chun-chun1, ZHU Cai-ying1, KAI Wen-long1, LIU Guo-jun2, LIU Qi-yong1, LIN Li3, QI Ai-ai3, WU Zhong-yi3, WANG Jun1, SONG Xiu-ping1, MENG Feng-xia1   

  1. 1 State Key Laboratory of Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, WHO Collaborating Centre for Vector Surveillance and Management, Beijing 102206, China;
    2 Weifang Center for Disease Control and Prevention;
    3 Hainan Health Pest Control Association
  • Received:2018-11-20 Online:2019-02-20 Published:2019-02-20
  • Supported by:
    Supported by the Major Special Fund Projects of the 13th Five-Year Plan (No. 2017ZX10303404002005), National Science and Technology Major Project of China (No. 2018ZX10101002-011) and the National Natural Science Foundation of China (No. 81273139)

海口市2018年白纹伊蚊击倒抗性基因型分布研究

赵春春1, 朱彩英1, 开文龙1, 刘国军2, 刘起勇1, 林丽3, 祁艾艾3, 吴钟毅3, 王君1, 宋秀平1, 孟凤霞1   

  1. 1 中国疾病预防控制中心传染病预防控制所媒介生物控制室, 传染病预防控制国家重点实验室, 世界卫生组织媒介生物监测与管理合作中心, 北京 102206;
    2 潍坊市疾病预防控制中心, 山东 潍坊 261061;
    3 海南省卫生有害生物防制协会, 海南 海口 570203
  • 通讯作者: 孟凤霞,Email:mengfengxia@icdc.cn
  • 作者简介:赵春春,女,在读硕士,主要从事蚊虫抗药性分子监测研究,Email:cczhaospring@163.com
  • 基金资助:
    “十三五”重大专项基金(2017ZX10303404002005);国家科技重大专项(2018ZX10101002-011);国家自然科学基金(81273139)

Abstract: Objective To investigate the mutations in the knockdown resistance (kdr) gene of dengue vector Aedes albopictus in Haikou, China, as well as genotypes and their distribution in different areas, to analyze the influencing factors on insecticide resistance for scientific control of Ae. albopictus. Methods In June and July, 2018, the larvae and pupae of Ae. albopictus were collected from the urban and suburb areas of Haikou and reared to adults. The collected individuals were identified by morphology and were cryopreserved at -20℃ with anhydrous ethanol. DNA was extracted from a single mosquito, partial fragments of the voltage-gated sodium channel gene on nerve cell membrane were amplified by PCR, and sequencing was performed to analyze the mutations in the kdr gene. Results In the 94 Ae. albopictus mosquitoes in the east, south, west, north, and middle of Haikou, mutation was detected at the F1534 locus of the kdr gene. There were four alleles at the 1534 locus, namely, wild-type TTC/F (113/60.11%), mutant TCC/S (66/35.11%), mutant TCG/S (1/0.53%), and mutant TGC/C (8/4.26%); there were six genotypes, i.e., homozygous wild-type F/F (39/41.49%), wild/mutant heterozygote F/S (33/35.11%) and F/C (2/2.13%), homozygous mutant S/S (15/15.96%) and C/C (1/1.06%), and mutant heterozygote S/C (4/4.26%). The proportions of genotypes varied between populations. Conclusion The kdr mutation rate is high in Ae. albopictus in Haikou. Local mosquito resistance should be further monitored, so that insecticides are used appropriately.

Key words: Aedes albopictus, Insecticide resistance, Knockdown resistance gene, Gene mutation, Haikou

摘要: 目的 了解海南省海口市登革热媒介白纹伊蚊的击倒抗性基因(kdr)在不同区域的基因型及其分布特点,并分析其抗药性产生的影响因素,为白纹伊蚊化学防控提供依据。方法 2018年6-7月在海口市东、南、西、北、中5个方位,采集白纹伊蚊幼虫及蛹,饲养至成蚊,经形态学方法鉴定后,用无水乙醇溶液浸泡,并于-20℃冻存备用。提取单只蚊虫的DNA,PCR扩增神经细胞膜上电压门控钠离子通道(VGSC)基因部分片段,测序后分析kdr基因突变情况。结果 对海口市不同方位采集的94只白纹伊蚊的检测结果显示,其kdr基因F1534位点存在突变。1534位点共有4种等位基因,即野生型TTC/F(113/60.11%),突变型TCC/S(66/35.11%)、TCG/S(1/0.53%)和TGC/C(8/4.26%);有6种基因型,分别为野生型纯合子F/F(39/41.49%),野生/突变型杂合子F/S(33/35.11%)、F/C(2/2.13%),突变型纯合子S/S(15/15.96%)、C/C(1/1.06%)和突变型杂合子S/C(4/4.26%)。各种群之间基因型比例各不相同。结论 海口市kdr基因突变率较高,应进一步监测当地蚊虫抗药性,合理使用杀虫剂。

关键词: 白纹伊蚊, 抗药性, 击倒抗性基因, 基因突变, 海口市

CLC Number: