Chines Journal of Vector Biology and Control ›› 2018, Vol. 29 ›› Issue (6): 601-603.DOI: 10.11853/j.issn.1003.8280.2018.06.012

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Investigation on Borrelia burgdorferi in rats captured at Kaihua county in Quzhou of Zhejiang province

WANG Shuang-qing, ZHAN Bing-dong, ZHANG Jian-min, CAO Guo-ping, ZHONG Jian-yue, YU Zhang-you   

  1. Quzhou Center for Disease Control and Prevention, Quzhou 324000, Zhejiang Province, China
  • Received:2018-08-13 Online:2018-12-20 Published:2018-12-20
  • Supported by:
    Supported by the Open Project of State Key Laboratory of Pathogen and Biosecurity (No. SKLPBS1450), the Project of the Medical and Health Science and Technology Plan of Zhejiang Province (No. 2016KYB332) and the Scientific & Technological Guidance Project of Quzhou City (No. 2015101)

浙江省衢州市开化县鼠类伯氏疏螺旋体感染状况调查

王双青, 占炳东, 张建民, 曹国平, 钟建跃, 余樟有   

  1. 衢州市疾病预防控制中心传染病防制科, 浙江 衢州 324000
  • 通讯作者: 占炳东,Email:26428423@qq.com
  • 作者简介:王双青,女,硕士,医师,主要从事传染病控制与疫情管理工作,Email:493440928@qq.com
  • 基金资助:
    病原微生物安全国家重点实验室开放课题(SKLPBS1450);浙江省医药卫生科技项目(2016KYB332);衢州市2015年度指导性科技项目(2015101)

Abstract: Objective The present study was conducted to investigate the infection of Borrelia burgdorferi in rodents in Kaihua county. Methods Nested PCR were used to amplify 5S-23S rRNA spacer fragments of B. burgdorferi from kidney samples of rodents captured by mouse cage in Kaihua county. And the positive samples were sequenced and analyzed. Results A total of 10 rodents were positive in 149 samples tested,with the prevalence of 6.71%. The positive samples were identified from Rattus edwardsi, Niviventer niviventer, N. fulvescens, R. tanezumi, Apodemus agrarius, and R. norvegicus. The predominant species in Kaihua county were Ap. agrarius, R. fulvescens, and N. niviventer and the positive rates were 4.05%, 10.00%, and 10.00%, respectively. The spleen specimens from the N. confucianus and N. fulvescens were positive for B.burgdorferi sensu stricto, and that from R. norvegicus was positive for B.valaisiana. Conclusion The N. confucianus and N. fulvescens are considered as important reservoirs. The infection of two B. burgdorferi genotypes in rodents was confirmed in Kaihua county. It is very important to enhance the B. burgdorferi surveillance in wild animals and focus the morbidity in population.

Key words: Borrelia burgdorferi, Rodents, Surveillance

摘要: 目的 了解衢州市开化县鼠类伯氏疏螺旋体的感染情况。方法 2015-2016年每年的4-11月在开化县选择不同生境,采用鼠笼捕鼠,采集鼠脾标本,巢式PCR用于扩增伯氏疏螺旋体5S~23S rRNA间隔区片段,并对阳性片段进行测序。结果 149份样本中检出阳性10份,总阳性率为6.71%,阳性鼠种有白腹巨鼠、北社鼠、针毛鼠、黄胸鼠、黑线姬鼠和褐家鼠,其中优势鼠种为黑线姬鼠、针毛鼠和北社鼠,阳性率分别为4.05%、10.00%和10.00%。测序结果表明,针毛鼠、北社鼠中伯氏疏螺旋体为B.burgdorferi sensu stricto,褐家鼠中伯氏疏螺旋体为B.valaisiana结论 开化县北社鼠和针毛鼠可能是莱姆病的重要宿主,鼠类存在2种不同基因型的伯氏疏螺旋体感染,应继续加强野生动物中伯氏疏螺旋体的监测,同时密切关注人群中莱姆病的发病状况。

关键词: 伯氏疏螺旋体, 鼠类, 监测

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