Detection of Pla Fragment in Yersinia pestis by two methods

doi:10.11853/j.issn.1003.8280.2017.03.010

Chines Journal of Vector Biology and Control ›› 2017, Vol. 28 ›› Issue (3): 238-240.DOI: 10.11853/j.issn.1003.8280.2017.03.010

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Detection of Pla Fragment in Yersinia pestis by two methods

JIN Mu-zi¹, YUN Hua¹, TAI Da-peng², CHEN Yu-fei¹, BAI Xiao¹

1 Inner Mongolia International Health Care Center, Hohhot 010020, Inner Mongolia Autonomous Region, China;
2 Baotou Medical College

Received:2017-02-21 Online:2017-06-20 Published:2017-06-20
Supported by:
Supported by the General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China Scientific Research Subject(No. 2015IK176)and the Natural Science Foundation of Inner Mongolia Autonomous Region of China(No. 2015BS0805)

两种方法检测鼠疫耶尔森菌Pla片段的研究

靳木子¹, 云华¹, 邰大鹏², 陈宇飞¹, 白潇¹

1 内蒙古国际旅行卫生保健中心, 呼和浩特 010020;
2 包头医学院, 内蒙古包头 014040

作者简介:靳木子,女,博士,主管技师,主要从事卫生检疫工作,Email:184022283@qq.com
基金资助:
国家质检总局科技计划项目（2015IK176）；内蒙古自然科学基金（2015BS0805）

Abstract

Abstract: Objective To determine the effectiveness of Southern blot method for detection of Pla fragment in Yersinia pestis. Methods Using Southern blot and Y. pestis quantitative real-time PCR kit to detect the Pla fragment. Results The results of Southern blot were same as real-time PCR kit. Southern blot allowed to see the number of pla gene copies directly, the efficiency of hybridization was high; PCR detection was also positive. Conclusion Southern blot is suitable for detection of Pla fragment of Y. pestis.

Key words: Southern blot, Quantitative real-time PCR, Yersinia pestis

摘要： 目的研究Southern印记杂交方法检测鼠疫耶尔森菌（鼠疫菌）Pla片段的有效性。方法采用Southern印记杂交技术与鼠疫菌核酸测定试剂盒两种方法对鼠疫菌Pla片段进行检测。结果两种方法检测结果相同，Southern印记杂交技术可直观地观察pla基因拷贝数，杂交效率较高；实时荧光定量PCR检测结果为阳性。结论 Southern印记杂交技术适用于鼠疫菌Pla片段的检测。

关键词: Southern印记杂交, 实时荧光定量聚合酶链式反应, 鼠疫耶尔森菌

CLC Number:

R254.8

JIN Mu-zi, YUN Hua, TAI Da-peng, CHEN Yu-fei, BAI Xiao. Detection of Pla Fragment in Yersinia pestis by two methods[J]. Chines Journal of Vector Biology and Control, 2017, 28(3): 238-240.

靳木子, 云华, 邰大鹏, 陈宇飞, 白潇. 两种方法检测鼠疫耶尔森菌Pla片段的研究[J]. 中国媒介生物学及控制杂志, 2017, 28(3): 238-240.

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Detection of Pla Fragment in Yersinia pestis by two methods

两种方法检测鼠疫耶尔森菌Pla片段的研究

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