Chines Journal of Vector Biology and Control ›› 2016, Vol. 27 ›› Issue (2): 206-208.DOI: 10.11853/j.issn.1003.8280.2016.02.030

• Review • Previous Articles     Next Articles

Application of high resolution melting curve analysis technology on the species identification and classification

HE Jiang1, YI Hai-hua2, ZHOU Peng-cheng3, CUI Bei-jin1, WU Bing-yao3, SUN Li-xin3   

  1. 1 School of Medicine, Anhui University of Science and Technology, Huainan 232001, Anhui Province, China;
    2 Xuzhou Entry-Exit Inspection and Quarantine Bureau;
    3 Jiangsu Entry-Exit Inspection and Quarantine Bureau
  • Received:2015-12-23 Online:2016-04-20 Published:2016-04-20
  • Supported by:

    Supported by the Science and Technology Project of the Jiangsu Entry-Exit Inspection and Quarantine Bureau (No. 2014KJ41, 2016KJ55)


何江1, 易海华2, 周鹏程3, 崔北金1, 吴炳耀3, 孙立新3   

  1. 1 安徽理工大学医学院, 安徽淮南 232001;
    2 徐州出入境检验检疫局, 江苏徐州 221000;
    3 江苏出入境检验检疫局, 南京 210001
  • 通讯作者: 孙立新,
  • 作者简介:何江,男,在读硕士,主要从事分子生物学研究工作,
  • 基金资助:



High resolution melting (HRM) curve analysis technology is a new method applied to genotyping and detection of gene mutations and single nucleotide polymorphism. The new method on the basis of the melting curve analysis, replaces the unsaturated dye with the saturation one, and with the aid of the professional high resolution instruments. HRM has received much concerns in the related fields for its advantages, such as high sensitivity and specificity, contamination avoidance, ease of operation, higher speed and flux, it has been widely used in the identification of insects, bacteria, fungi, protozoa and the classification of virus.

Key words: High resolution melting, Species identification, Classification



关键词: 高分辨率熔解曲线, 物种鉴定, 分型

CLC Number: