Chines Journal of Vector Biology and Control ›› 2014, Vol. 25 ›› Issue (4): 294-296.DOI: 10.11853/j.issn.1003.4692.2014.04.002

• Original Reports • Previous Articles     Next Articles

Evaluation of dilution rose bengal plate agglutination test in diagnosis of brucellosis

SUN Yan1,2, XIAO Pei2, WANG Na1,2, ZHAO Na2, PIAO Dong-ri2, TIAN Guo-zhong2, ZHAO Hong-yan2,JIANG Hai2, CUI Bu-yun2, DU Ya-nan1   

  1. 1 College of Veterinary Medicine,Inner Mongolia Agricultural University,Hohhot010018,Inner Mongolia Municipality, China;
    2 State Key Laboratory for Infections Disease Prevention and Control,Collaborative Innovation Center for Diagnosis and Treatment of Infections Diseases,National Institute for Infectious Diseases Control and Prevention,Chinese Center for Disease Control and Prevention,Beijing102206, China
  • Received:2014-03-24 Online:2014-08-20 Published:2014-08-20
  • Contact: DU Ya-nan, Email: yanandu@126.com; CUI Bu-yun, Email: cuibuyun@icdc.cn
  • Supported by:
    Supported by the 12th Five- Year Major National Science and Technology Projects of China(No. 2011ZX10004-001,2012ZX10004215)and World Bank-financed Projects(No. TF12401)

倍比稀释虎红平板凝集试验对布鲁氏菌病诊断的探讨

孙岩1,2,肖培2,王娜1,2,赵娜2,朴东日2,田国忠2,赵鸿雁2,姜海2,崔步云2,杜雅楠1   

  1. 1 内蒙古农业大学兽医学院,呼和浩特010018;
    2 中国疾病预防控制中心传染病预防控制所,传染病预防控制国家重点实验室,感染性疾病诊治协同创新中心,北京102206
  • 通讯作者: 杜雅楠,Email: yanandu@126.com;崔步云,Email: cuibuyun@icdc.cn
  • 作者简介:孙岩,男,在读硕士,从事布鲁氏菌病相关研究,Email: 297553262@qq.com;肖培,男,在读硕士,从事传染病流行病学研究,Email: woodyco@sina.com
  • 基金资助:
    国家“十二五”科技重大专项课题(2011ZX10004-001,2012ZX10004215);世界银行资助项目(TF12401)

Abstract: Objective To explore the feasibility of dilution rose bengal plate agglutination test (RBPT) for the diagnosis of brucellosis.Methods Serum samples from 93 brucellosis patients who came from Inner Mongolia, China in 2013 were tested by both dilution RBPT and standard tube agglutination test (SAT). According to the correlation between the results of the two tests and using the results of SAT as criteria, the sensitivity and specificity of RBPT were analyzed at different dilutions as cut-off values to evaluate the diagnostic accuracy.Results The area under the receiver operating characteristic curve for dilution RBPT was 0.946. When taking1∶2 as the cut-off value, the Youden index was 0.893, the sensitivity was 89.30% , and the specificity was 100%; when taking1∶4 as the cut-off value, the Youden index was 0.631, the sensitivity was 63.10%, and the specificity was 100%; when taking1∶8 as the cut-off value, the Youden index was 0.571, the sensitivity was 57.14%, and the specificity was 100%; when taking1∶16 as the cut-off value, the Youden index was 0.191, the sensitivity was 19.05%, and the specificity was 100%.Conclusion Dilution RBPT has the highest diagnostic accuracy at a dilution of1∶2, which provides a reference for the diagnosis of brucellosis.

Key words: Diagnosis of brucellosis, Rose bengal plate agglutination test, Tube agglutination test, Cut-offvalue

摘要: 目的 探索倍比稀释虎红平板凝集试验(RBPT)作为布鲁氏菌病(布病)诊断实验的可能性。方法 选择2013年内蒙古自治区布病患者血清93份,同时进行试管凝集试验(SAT)和倍比稀释RBPT检测,根据二者实验结果的相关性,以SAT结果作为判定标准,分析不同稀释倍数的RBPT作为诊断截断值的灵敏度和特异度来评价诊断的准确性。结果 倍比稀释RBPT受试者工作特征曲线下面积为0.946,以1∶2为截断值时约登指数最高为0.893,灵敏度为89.30%,特异度为100%;以1∶4作为截断值,RBPT的灵敏度为63.10%,约登指数为0.631,特异度为100%;以1∶8作为截断值,RBPT的灵敏度为57.14%,约登指数为0.571,特异度为100%;以1∶16作为截断值,RBPT的灵敏度为19.05%,约登指数为0.191,特异度为100%。结论 倍比稀释RBPT以1∶2为截断值时诊断的准确性最高,可以为布病诊断提供参考。

关键词: 布鲁氏菌病诊断, 虎红平板凝集试验, 试管凝集试验, 截断值

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