In this paper two new species and a new record of the genus Megophyra Emden,1965 are described from western mountainous region of Sichuan Prov.,China.The type and new record specimens are preserved in the Medical Entomological Museum,Institute of Microbiology and Epidemiology,Academy of Military Medical Sciences,Beijing,China.1 Megophyrapedanocerca sp.nov.(Fig.1)♂:Body length 9.0mm.This new species is similar to Megophyra mimimultisetosaFeng,2000,but differs from the latter in having theac3+1,dc 2+4,male surstyli back-curved and postmargin sunken on apical part in lateral view;cerci very short,length about 1.7 times of its width on middle part in lateral view.♀:Unknown.Holotype♂,Mt.Erlang Forest Farm(2 690m,29°53N,102°10E),Sichuan Prov.,8,Jul.,1984,taken by Feng Yan.2 Megophyra nigritibia sp.nov.(Fig.2) ♂:Body length 9.0mm.This new species is similar to Megophyra fuscitibiaEmden,1965,but differs from the latter in having the ac2+1;interfrontalia present;male cerci narrower on posterior view;5th sternite lateral lobe inward on apical part in ventral view.♀:Unknown.Holotype ♂,Mt.Erlang Forest Farm(2 680m),Sichuan Prov.,1,Jul.,1988,taken by Feng Yan.3 Megophyra fuscitibia Emden,1965 N.R.(Fig.3)The materials of the newly recorded species have been collected from Mt.Erlang of Sichuan,China.It was originally reported from Burma,some further notes on characters of male are given in the Chinese text.

Two new species of Dasyhelea were collected by the authers from Zhuhai City,Guangdong Province: Dasyhelea dioxyria sp.nov.and Dasyhelea microsporea sp.nov.The type specimens are deposited in the Medical Entomology Collection Gallery(20 Dongda Jie,Fengtai,Beijing 100071,China).

Objective:To investigate the variation of the population density of Bandicota indica.Methods:On the basis of the field investigation in the flatland areas in Ruili city and Longchuan county from 1988 to 1996 and the related investigation material from other districts in Yunnan province,the variation of the population density of Bandicota indicawas analyzed in this paper.Results:The results revealed that Bandicota indica was comparatively concentrated in the flatland areas with 1 100m of altitude in Ruili and Longchuan,which reflexed a strong characteristics of limited areas and altitude.The seasonal fluctuation of the population density all showed a three peak type in the two flatland areas,the emerged time from January to April,June and from November to December in Ruili.But it's from January to April,August and from November to December in Longchuan.The annual variation of nine years(12 months per year) showed that the constituent of Bandicota indicain the small mammal community was relatively stable in the two areas.Conclusions:Bandicota indicamust be one of the plague host animals.

Objective:To screen the plants that have chronic toxicity to rodents as well as are safe for untargeted animals and for environment.Methods:A series of experiments were carried out in laboratory.Albino mice were feed with pulverized plants directly or diet which mixed with pulverized plant powder and flour.Several plants were screened for next trial according to the mortality.Then,the animals were feed with the food that was mixed the extracting plant solutions with normal food.Results: Nicotiana tabacuw and Nerium indicum were poisonous to mice.Conclusions:The mortality shows that tobacco has the potential to be used to kill rodents.

Objective:To develope antifreezing and dry proofing pasty rodenticide.The composition and preparation of the antifreezing pasty rodenticide were reported.Methods:Using 0.15% Bromadiolone as rodenticide,20%-40% polylol as antifreezing agent,1%-2% PMD-300 as pasty agent.Results:The pasty rodenticide can be used at temperature from -35℃ to 50℃.Conclusion:It has satisfactory killing rate for rats and can be used at low temperature circumstance for killing sly rats.

Objective:To analysisthe quality of rodent control with poison baits in apartments on the different streetsin a process of once rodent control.Methods:Direct observing and questionary.Result:The rate of cast bait was 87.56%,82.09% and 75.33% respectively.The rate of rodent trace was 16.59%,19.02% and 21.72% respectively.Thequalified rate for rodent proof equipment was 78.99%,68.75%,52.86%,respectively.The rate of effect was 81.41%,55.37% and 55.74%in order.Conclusions:The resultindicated that rodent control in the street is un-balanced.The rodent control of around street should bestrengthened.

Objective:To compare the sequence difference of the ribosomal DNA second internal transcribed spacer(ITS2) in the different strains of Anopheles minimusin Yunnan.Methods:The PCR amplified rDNA ITS2 sequences,to compare the variation of An.minimus.Results:The different strains of 6 populations were sequenced,which could be separate into Anopheles minimusA and C.Conclusion:The sequencedifference of the rDNA ITS2 would be very useful for the developing a molecular approach to distinguish An.minimusA and C.

Objective:To study on the mosquito communities and the geographical niche of border area inYunnan province.Methods:Alluring by man body in day time and traping by trapper at night.Results:The mosquito communities were investigated in 15 border counties in Yunnan.The results showed that there were 6 dominant species of night-actived mosquitoes around domestic animal and human house in the rural area and 5 dominant species of day-actived mosquitoes in the groves of bamboo and woods.They were: Culex tritaeniorhynchus,Culex pseudovishnui,Culex fuscocephala,Culex whitmorei,Anopheles sinensis,Anopheles vagus,Aedes albopictus,Aedes pseudabopictus,Aedes annandale,Aedes vexans and Armigeres subalbatus.The diversity indexof night-actived mosquito communities were:Cangyuan(2.092 0),Ruili(2.035 5),Yingjiang(1.9521),Jinghong(1.8193),Maguan(1.7234),Genma(1.6857),Mengla(1.6717),Jingping(1.6709),Shuangjiang(1.6488),Menglian(1.6444),Menghai(1.568 8),Wangding(1.4616),Hekou(1.427 1),Luxi(1.2940) and Baoshang(1.2364).The diversity indexof day-actived mosquito communities were:Hekou(2.1630),Jinghong(1.9355),Mengla(1.7447),Cangyuan(1.6756),Genma(1.6734),Yingjiang(1.625 8),Luxi(1.601 5),Wangding(1.594 8),Ruili(1.531 8),Menglian(1.161 9) and Menghai(1.056 1).Conclusion:Thebiggest geographical niche breadth to night-actived mosquitoes wasCulex pipiengsquinquefasciatus(0.858 6),Culex tritaeniorhchus(0.838 2),Culex pseudovishnui (0.8015) and the biggest geographical niche breadth to day-actived mosquitoes wasAedes albopictus(0.895 5).

Objective:To observe the effectiveness of Responsar SC125 against Anopheles anthropophagus.Methods:Absolving the bednets with Responsar ^?solution.The bioassay test of Responsar SC(Beta-cyfluthrin 12.5% w/v) against Anopheles anthropophagus in laboratory was carried out during 1997-1998.Results:The effects of Responsar against Anopheles anthropophagusdepended on the active ingredient of Responsar,as well as the contacting time and test methods.Increasing active ingredient or extending contacting time will improve the effect.Conclusions:At the same step of the test,using same time and same materials,the nets impregnated with Responsar washed with clear water were less effective than the nets unwashed.

Objective:To research for closure type mosquito coil incense utensil.Methods:To detect the dust,incense utensil and volatile noxious.Results:This paper reported that both the closure type and the opening type mosquito coil incense utensil.The volatile noxious to be one's health of the matter,decrease 67.49%,u =9.42,P<0.05;≥0.5μmdust 67771.75/Land 71811.84/Ldecrease5.63%,χ ²=40.77,<0.05;≥0.5μm dust as time going on was decrease, r=0.9888,t=11.47,P<0.05;simulation localetestresults of the biometrics,unconsciousnessrate:1hour 32% and 28%,3 hour both the closure type and the opening typemosquito coilincense utensil100%.

Objective:To study on the composition of fly species in Jinhua city area.Methods:Regular catching method.Results:Thecomposition of fly species was observed and analyzed in Jinhua city area from June to November 1998 and from March to November 1999.The flies in Jinhua city area included 13 species belonging to 10 genera among 4 families.Among them, M.sorbens,C.megacephala,L.seriata and L.cuprina were superiority species.Conclusion:The compositions of fly species in different years,months,habitats and places were different.

Objective:The resistances of house flies could be controlled by the way of the comprehensive controlling measures and the changed or mixed employment of insecticide,And then the effect of insecticide could be enhanced.Methods:The surveillance net has been built,and the changes of resistance on flies to insecticide were measured by dropping-test-way.Result:According to the data of resistance on flies form1997 to 1999,it was observed thatLD ₅₀ of DDVP,permethrin,deltamethrin were0.1854,0.0409,0.0077(μg/per.insect) respectively,Which was 3.94,1.03,4.65,1.98times higher than that of the years from 1982to1985,7.00and 3.5times higher than that of the years from 1991 to 1992.LD ₅₀ of cypermethrin to house flies were varied from0.0390to0.0774μg per-insect at the basis of cypermethrin resistances surveillance on fly.Conclusion:It was indicated that rational employment of insecticides could enhance effects on house flies and inhibit or delay developing of the resistances on house flies.

Objective:To observe the efficacy of 10% FENDONA SC against mosquito,housefly and cockroach.Methods:Force contacting method.Results:The efficacy for the three insects was the best on the glass surfaces.Efficacy on the cement was better than the painted wood.The efficacy for mosquito was the best on the three surfaces.The efficacy for cockroach was better than the housefly.The long-lasting efficacy for mosquito all was over 12 weeks on the three surfaces;The long-lasting efficacy for housefly all were 12 weeks on the glasses,while on the painted wood 10mg/m ²about 7 weeks,20mg/m ²about 8 weeks,30mg/m ² about 10 weeks,while on the cement 10mg/m ² about 10 weeks,20mg/m ²and 30mg/m ² exceeding 12 weeks;The long-lasting efficacy for cockroach exceeded 12 weeks on the glasses,on the painted wood and cement surfaces 10mg/m ² about 10 weeks,while 20mg/m ²and 30mg/m ² over 12 weeks.

Objective:To study the susceptibility or resistance ofGerman cockroach,including three field-collected strains in naval vessels fromDalian(Rp),Shanghai(Rc)and Zhanjiang(Rz)to cypermethrin,deltamethrin and propoxur.Methods:The method of contact with chemical cover was used in this study.Result:The average resistance ratioes offield-collected strains to cypermethrin were1.84,1.75,1.92 respectively;those of field-collected strains to deltamethrin were 2.44,2.82,1.15 respectively;those of field-collected strains to propoxur were0.89,1.27,1.36 respectively.Conclusion:Three strains of German cockroach have already developed varied low resistance to the three kinds of insecticides.

Objective:To explore the cockroach control strategy at top-grade railway passenger station by observing the species,habitats of the cockroach and studying the effects of varied cockroach-killing methods at different sites.Methods:The integrated procedures included principal environmental improvement together with intensifying survey,appropriate cockroach-killing ways in different sites and focusing on the key places.Result:The cockroach density reduced by77.99%～90.26% and77.48%- 98.24% at different surroundings,respectively in 1998 and 1999 in comparison with that of 1997's,before applying the integrated procedures,and the ovum density dropped to 0-0.03and0respectively.The cockroach density of some sites cut down on66.67%-88.89%,74.11%-88.00%,70.00%-79.07% and 70.59%-75.71%,respectively at3,7,15 and 30 days after spraying pesticide as comparing with that of when cockroach-proof steps hasn't been taken.The pivots cockroach density was1.16-1.30and0.38-1.18times the average while strengthening the cockroach killing of pivots in comparison with 2.38-2.93times of the contrast.At the same time the amount of insecticide decreased and the fund was saved.Conclusion:Promising efficiency on cockroach control was gained at top-grade railway passenger station,some social and economic benefits were obtained.

Objective:To reduce the use of organic solvent and to control the release of efficiency.Methods:Refer to efficacy test concerned methods of public health insecticides for registration,on the basis of taking high-molecular compound as control release formutation and substituting organic solvent for distilled water,2.5% Control release micro-emulsion of Deltamethrin has been developed.Result:Theinsecticide knocked mosquito and fly down quickly,and killed them powerfully well-matched as 2.5% Decis;which used as delaying spray hasefficiency as long as 2.5% K-othrine nearly.Conclusion:It's low toxicity company good stability either chemical or physical.It can be taken as an effective spray.

Objective:Trial-produce slow relesase repellent with inclusion compound technology.Methods:The deet inclusion compound was made in method of saturated solution.Its toxicity was tested by using method of GB7919-87.Repelling effect of human body was tested by testing methed of GB/T17322.10-1998.Result:Its oral and dermal LD ₅₀were over5000mg/kgand3000mg/kg respectively in rats,efficacious time was 6.2±0.54 for human body,it has no dermal and eye irritancy on rabbits.Conclusion:The deet inclusion compound has almost no toxicity for human boday,it was very steady on light and heat,the active in gredient were slow relesase.

Objective:To evaluate repellent efficacy,fungistatic efficacy and safety of the camphor oil spray.Method:Place the human volunteer's hand treated with the camphor oil inside a cage containing 300 Aedes albopictus,for 2 min,and observe the treated skin surface for mosquitoes which landed and attempted to feed.Mingle the camphor oil and the fungous suspension,Candida albicans,culture and count the number of mold.Infuse the mice with the spray by single oral injection,and treat directly the rabbits'exposed skin and the conjunctiva crypt with the spray.Result:The repellent protection time against Aedes albopictuswas over 6h.Mean fungistatic rate to mold was99.99%.The acute oral LD ₅₀ was 1302.5 mg/kg in mice.This product was non-irritant to skin,but slightly irritant to eye.Conclusion:The camphor oil spray is a better repellency against mosquito,inhibitor against mold,and low acute toxicity substance.

Objective:To observe the efficacy of Allbest Wp4.5 agamst mosquito,housefly and cockroach in lab.Methods:Force contacting method of GB.Results:The efficacy tests of Allbest Wp 4.5 were made against three public health insects in laboratory.On the glass surface of all the three,the application quantity was the least,the efficacy was the best,the long -lasting efficacy was 90 days for the three insects.Among the three kinds of insects,the best efficacy was obtained against the housefly.Conclusions:Allbest Wp4.5 has satisfactory efficacy against the public health important insects.

Objective:To observe the neutralizing antibody reaction after secondary immunization with Bivalent inactivated vaccine for epidemic hemorrhagic fever.Methods:Some volunteers were injected with bivalent inactivated vaccines for epidemic hemorrhagic fever(HFRS).3 doses were for primary immunization and one dose for secondary immunization after one and half year's interval.Neutralizing antibodies(NA)against HTNV and SEOV in sera before and after secondary immunization were detected by a plaque reduction neutralization test(PRNT).Result:TheGMT of NA against HTNV and NA against SEOV after secondary immunization were 1:28.29 and 1:32.49,respectively.The GMT of NA increased(3-10 fold)compared with that before secondary immunization.

Objective:To understand clinical characteristic of tsutsugamushi disease occurred in Hebei province.Methods:General check-up for all the cases were carried out in scene of the epidemic focus.All the patients were diagnosed by serological method tested to be positive of IgM antibody to tsutsugamushi disease.Result:Average temperature of the patients was 39.7℃,and fever-duration wes9.0days.Among 38 patients,30 cases(78.95%)with eschar,22 cases(57.89%) with rash and 34 cases(89.47%)with lymph node enlargement were found.29(96.67%) of30cases with eschar were one eschar per case.The eschars were mainly distributed on waist,groin,inside of thigh and so on.Rash appeared 1 st to 8th day after attack,and duration wes 2-8days(average4.5days).Most of systems of the body were implicated,and 4cases(10.5%)were complicated by pneumonia,encephalopathy,encephaledema,heart failure and toxic shock.Conclusion:Clinical symptoms and manifestations of the most cases were typical.But a part of cases need diagnosis with serological methods.