To investigate the biological properties of Mus musculus, snap-trapping was used in the middle ten-days of every month from April to October during 1991 to 1996 in different habitats of Ordos sandland. The results showed that Mus musculus was uncommon species which accounts for only 2% of the total rodent number in the area. The optimum habitats of Mus musculus were low-lying lands among dunes, while wind-drifed dunes were the most unsuitable habitats. A total number of 162 specimens were collected and dissected. All the specimens were divided into four age groups based on their body weight excluding viscera. The body weight excluding viscera was ≤6.9g for group Ⅰ (juveniles), 7.0～8.9g for group Ⅱ (subadults), 9.0～12.9g for group Ⅲ(adults) and ≥13.0g for group Ⅳ(old ages). Their breeding period was from March to October and litter size was 7.08±1.90.

The paper introduces the investigations of Rattus norvegicus on susceptibility and resistance to 0.005% Warfarin bait in Jinan Military Area where anticoagulant rodenticide have been used for many years. It indicated that resistance incidence of R.norvegicus was 2.04% in Jinan city, and 4.17% in Xuchang city while R.norvegius were still susceptible to anticoagulant rodenticides in Weifang city and Huixian city. In the laboratory, all of the killing rates with “un-selective ingesting”were all 100.00% to Indian corn bait containing 0.005% Bromadiolone, 0.025% Racumin and 0.025% Diphacine-Na, killing rate with “selective ingesting”were 92.86% in Bromadiolone bait, 61.54% in Racumin, and 38.46% Diphacine-Na. Ingesting index was 0.70 in Bromadiolone bait, 0.45 in Racumin and 0.22 in Diphacine-Na. In the field trial, the palatability of Bromadiolone and Racumin rice baits to R. norvegicus were better than Diphacine-Na and the control deratization rates were 85.11%, 87.33% and 81.55% respectively.

1 ppm and 2ppm NRDC ₁₆₁ can protect grain bait 100 percent from harmful insects for 12 monthes indoor. The breeding index of white rat for 1 ppm and 2 ppm NRDC ₁₆₁ maize and wheat bait were 0.93 and 0.8 in laboratory and it was not toxicity for rats. The breeding index which 1ppm NRDC ₁₆₁ grain bait to be eaten by rats was more than 0.96 in line of food and residence area. 5/10 000 Diphacine-Na ordinary poison bait and anti-insect poison bait were used to kill rats in spot. The deratization rates were 91.29 percent and 88.69 percent. There isn't differece between the former and the latter by statistic test.

The content of 6 kinds of elements in mosquito body of Aedes aegypti in different growth stage including the female mosquitoes newly-emerging and 0d, 5d, 10d, 15d after taking blood meal were detected and compared by ICA-P400. The results showed that there was remarkable significant difference in the 6 kinds of elements during different growth stage.

Aim: Susceptibility to 15 insecticides of deltamethrin-resistant Culex pipiens pallens was determined to utilize the existing insecticides reasonably. Method: Larva soaking method was used. Results: The mosquito's resistant ratioes to DDT、BHC、DDVP、trichlorphon、phoxim、malathion、fenitrothion、dioxacarb、propoxur、BPMC、cypermethrin、allethrin、phenothrin and resmethrin were respectively 6.80,8.92, 0.90, 1.18, 0 96, 1.29, 1.20, 1 60, 2.27, 1.51, 20.89, 23.14, 12.45, 58.71 and 36.62. Conclusion: If the mosquito yields resistance to deltamethrin, exchange or mix use of organophosphate or carbamate insecticides may be a better method.

The cross-resistance of 3 resistant strains of Cx. pipiens pallens to 5 kinds of chemical insecticides was studied. The results showed that DDVP-resistant strain was crossly resistant to propoxur and cypermethrin, propoxur-resistant strain was crossly resistant to DDVP and cypermethrin, and cypermethrin-resistant strain was crossly resistant to deltamethrin and DDT. Negative cross-resistance was found in DDVP-resistant strain with DDT, propoxur-resistant strain with deltamethrin. The results provided the insight for the rational use of chemical insecticides.

Seven field-collected strains of the housefly, Musca domestica, were tested for resistance to 4 different insecticides, DDVP, Bassa, Permethrin and Deltamethrin by the topical applications method in comparison with a known susceptible strain. The LD ₅₀ of DDVP, Bassa, Permethrin and Deltamethrin for the houseflies were 0.2507～0.5382, 12.5803～94.5318, 0.0519～0.4160 and 0.0111 ～0.0346μg/♀ respectively. Resistance ratios ranged from 0.15 to 3.58 in 1991, and increased 3.70 ～25.28 times in 1996. It was discussed that the management strategies of resistant houseflies in Guangdong Province.

In order to rapidly detect the resistant level of housefly, to organophosphate or carbanate insectides. The individual esterase activity was measured by filter paper test. The results showed that the esterase activity increased in the DDVP or fenobcarb resistant strains but didn't raise in deltamethrin resistant strains. There were positive relativity between esterase activity and resistance ratio to DDVP or fenobcarb. The inhibitory frequency of DDVP or fenobcarb to esterase activity in the susceptible population was evidently higher than that of the DDVP or fenobcarb resistant strains.

Purpose: To solve the key problem of improving the effect of luring and killing fly with luring fly toxicant bait. Method: The luring and killing effect of six toxicant baits and milk powder were tested respectively and repeatedly in lab and on spot. Result: Flies can be lured and killed quickly by No Ⅰ、No Ⅵ toxicant bait and milk powder. The number of lured and killed flies with milk powder was more than that with baits during four different watching time in ten mimutes. Six types of baits had quite different rates of killing effect in four hours. The maximun rate of killing was 56%. The minimum rate was 16%. But these rates were not ideal (The ideal rate is 85%). Conclusion: In order to promote the rate of killing flies reforming the prescription and process technology of the baits are needed.

This paper reports the categories of cockroaches from Hebei cities and their regional distribution. From 1996 to 1998, we trapped cockroaches with wide-mouth flasks in 11 cities under the jurisdiction of provincial government. There were 8 species belonged to 4 genera of 4 families. B.germanica was the first predominated species, and the next was P.japonica. The cockroahes in cities had the characteristics of block distribution and spot distribution.

The bacteria carried on the surface of cockroach in Hebei province was investigated. The cockroach captured in 5 cities was washed with 1% peptone solution. The solution was subsequently inoculated into 4 bacteria reproductive solutions. The bacteria were identified routinely. The results showed that 96.77% of cockroaches carried bacteria. Forty-nine strains of pathogenic or opportunistic pathogens were found. These results verified that cockroaches are of high bacteria positive rate on its surface. Its role on the disease transmission should not be ignored.

This paper reported the manufacture of DDVP microencapsulation preparation and continuing effect of killing insect. Four DDVP crude oil、acacia、gelatia and polyviny alcohol was used to synthetise the preparation by using compound coacervation method in laboratory. The preparation was composed of microencapsulation、suspending agents and water. The larva of Culex pipines pallens was used in the experiment of the microencapsulation preparation and emulsion's killing insect effect. Finally result, the residual effect of the microencapsulation preparation was longer than the emulsion.

Objective: The study of a kind of poison bait for ant and cockroach was reported. Methods: The poison bait was made up with acephate and permethrin (proportion 10∶1) cotoxicity coefficient (CTC) was 127; LD ₅₀ by oral and skin were all 5 000mg/kg for mouse; The time of 100% killing rate for cockroach was 72 hours and for ant was 102 hours. Conclusion: This bait composition is a reasonable priscription which have the advantage of high efficacy, low toxicity and easy to use, So it is worth applying in large scale.

Objective: Cohort study of seroepidemiology on inapparent infection of HFRS and antibody variation after inapparent infection of HFRS in control group was carried out in a randomized controlled field of type Ⅰ inactivated vaccine against HFRS. Methods : Indirect immunofluorescent antibody method was used in testing IgG antibody in sera, seroconversion to HFRS antibody and antibody variation after inapparent infection of HFRS in control group were stratified analysis by sex and age groups. Results: After 40 months followed-up in 360 persons (1994.7～1997.11), year rate of seroconversion to HFRS antibody was 2.93%. There were no statistically significant difference in positive seroconversion rates of sex and age groups; In 32 persons of inapparent infection of HFRS, negative seroconversion rate of antibody to HFRS was 46.88% and rate of antibody increase to HFRS was 25.00%, also there were no statistically significant difference in negative seroconversion rate of antibody and rate of antibody increase of sex、age groups and different HFRS antibody titer. Conclusion: The results showed background data to evaluate the field protection efficacy of an inactivated HFRS vaccine.

A seroepidemiological investigation of Lyme disease was carried out in 155 residents in forest area of Shanggao county of Jiangxi province. The results showed that 10(6.5%) residents had positive IgG titers (1∶128) against Borrelia burgdorferi6(3.9%) residents were patients with clinical Lyme disease. Sera of 110 goats and 62 cattles lived in forest area were tested by ELISA, their positive rate of IgG were respectively 19.1% and 12.9%. The results suggested that the Shanggao county maybe the epidemic area of Lyme disease.

We investigated on species composition, distribution, wax and wane of Ixodoidea and Isolated from Lyme disease spirochetes in eight cities and counties of Jiangsu province. We found that Ixodes distributed in south and north of Yangtse river. 1 family 2 genera and 4 species had been known in this area. Ixodes population varied in vary seasons. They appeared in April, got top in May and June, slowly dropped down in August, and disappeared in October every years. No Lyme disease spirochetes had been isolated from Ixodes.

Parasitism of demodex has something to do with skin disease and brandy nose, this has been proved by many scholars. We investigated the parasite on some residents whose age ranged from one to seventy. We used transparent adhesive paper glue to observe the infection of demodex on healthy face people and brandy nose patients. The result showed that the parasitism of demodex connected with brandy nose and the age. The older, the more infection with demodex. Compared with former decades, the rate of demodex infection has been much reduced.