The genomic DNAs of three species of Aedes ( Aedes albopictus、 Ae.aegypti 、 Ae.togoi）were amplified by RAPD-PCR technique with three primers (P _z、 P _w、 P _L).Different maps were obtained.Most of the amplified fragments are unique to one species, and some of fragments are common forthree species.These results indicate that the RAPD-PCR technique may be useful in studies of mosquitomolecular taxonomy.for its simplification、rapidness、and exactness.

This paper analyses the relationship between the geographical distribution of An.thropophagus and environmental factors in Sichuan Province systematically.Many environmental factorsare concerned: hydrographic net, human population density, farming land type, soil texture and altitudeabove sea level.1.Mostly, An.anthropopagus was distributed at the areas of the Minjiang Valley, theChangiiang trunk drainage in the southern part of sichuan Basin and the Tuojiang Valley.The ratio of An.anthropophagus in human dwellings showed some differences among these three water systems:53.86% and 61.90% and 22.74% respectively.2. An.anthropophagus was most widely spread in theareas with a human population density of 100 ～ 600 people per square kilometer from 1951 to 1990.During 1971 ～ 1980, An.anthropophagus in human dwellings was mainly distributed in the areas of humanpopulatuion density more than 600 people per square kilometer with a ratio of An.anthropophagus ashigh as 45.71%.3. An.anthropophagus was largely distributed in new alluvial soil, Chengdu clay, yellowand purple soil; only some in brown-podzol earth, marshy soil, mountain-meadow soil and podzolic brownforest soil.It seems that the distribution of An.anthropophagus is in relation to soil texture, but the soiltype is not a decisive factor.4. An.anthropophagus was chiefly distributed in plain, hilly land and lowmountain less than 1000 meters above sea level, where there are irrigated fields during winter.No An.anthropophagus was, however, found at the altitude above 1000 meters and dryland areas.The resultsshowed that the geographical distribution of An.anthropophagus was significantly influenced by the altitude above sea level and the existence of irrigated field.Nevertheless, some cities (ortowns) with irrigatedfields still had no An.anthropophgus found below the altitude of 1000 meters.Hence, besides the factors mentioned above, other factors such as meterological factor may play some important role in the geographical distribution of An.anthropophagus.5.Malaria incidence in the areas with An.anthropophagus had a close relation to the ratio of An.anthropophagus in human dwellings.In the MinjiangValley and the Changjiang trunk drainage, the ratio of An.anthropophagus was much higher than it inthe Tuojiang Valley, Jialing Valley and the Wujiang Valley.Proportionally, malaria incidence in the former areas was also much higher than it in the latter areas.In addition, based on the density of populationliving in the areas with An.anthropophagus, the denser the population, the lower the incidence and viceversa.Reasons are perhaps due to differnt economic level, cilivization degree and public health care between the two groups.

This article reported a laboratory evaluation of kerosene lamp containing syntheticpyrethroids to repel Anopheles dims.These test groups were control, kerosene lamp only and kerosenelamp containing 0.05% d-allethrin.By comparation of biting frequency of Anopheles dims among thethree test groups, it was confirmed that the obvious effect of kerosene lamp containing 0.05% d-allethrinto repel Anopheles dims in the laboratory, which also can kill some Anopheles dims during the test.Thetime distributions of biting by Anopheles dims in the three test groups were also analysed.

The bioassay on mosquitoes, flies and cockroaches through tests in laboratary showed thatthe synergism of FB-1 Shachong Wang was very significantly.As a insecticide, FB-1 Shachong Wang is considered great exploiting prospects.

The results showed that the main flies species of waste disposal was housefly which accounted for 99.16 per cent.The flies which were carried by the garbage vessels were one of the reasonsthat caused flies population density increasing on the wharf and disposal site.The results were a proofthat the fundamental control measure was covered effectively with slush on the waste disposal site.

By observations on the bionomics of Periplaneta japonica, we discovered that duringJune to August its population density is the highest in northern China.In June it is imago and in Augustit is mainly larvae.By the field insecticide experiment, we found that the optimal time for effective control of Periplaneta japonica is in May to June in northern China.

This paper firstly reports the studies on ksryotype of chromosomes by air-daying technique in Ornithonyssus bacoti.The results showed that O.bacoti had 8 (mal), 16 (female), 32, 64, and128 chromosomes.The paper firstly provided a useful method, air-drying technique, for studing chromosomes of mites;and firstly provided supernumerary chromosomes in mites.

The pharyngenal armatures of the sandflies are one of the most important taxonomiccharacters.In this paper, four groups of phsryngenal armature in female Phlebotomus chinensis collectedfrom different regions were studied by scanning electron microscopy method.The observation showedthat there were no obvious differences in the four groups and these sandflies belonged to one species.

Parasitic arthropods in the flock of sheep in Shanxi Province were investigated and analysed from May, 1992 to June, 1994.The investigated areas consisted of 1389 villages or towns of 108counties or cities in Shanxi province.1340 sheep and 1466 goats were inspected.It was demonstrated thatthe parasitic arthropods consisted of 19 species belonging to 7 families.The dominated species were Oestrus ovis and Sarcoptes scabiei var. ovis in the whole province, Dermacenter nuttalli in the north of Shanxi, Sarcoptes sabiei var. caprae, Psorptes equi var. ouis, P. equi var. caprae, Haemaphysalis uerti- calis, Hyalomma Hyalomma detritum, Boophilus Hyalomma rufipes and Ixodes pomeramtze- vi in proper order in the south of Shanxi.In addition, some new species, e.g. Hippobosca (underfinedspecies), Linguatula (underfined species), Haemaphysalis bispinose and Hyalomma detritus albipictum were found in Shanxi province.In the south of Shanxi, warmer weather, lower elevation, more rainfall,and more species of medical arthropod than the others.

A monitoring program of commensal rodent infestation has conducted for eleven years inBeijing.The monitoring result showed that there are two species of commensal rodents in Beijing.Theyare Rattus norvegicus and Mus musculus.The former isdominant in rural, the latter is dominant in urban.It showed that there are two density peaks for both commensal rodent (between February-April andSeptember-November).Since 1986, the regularity has not changed because of extensive commensal rodent control campaign which has made the density of commensal rodent to decline obviously.The incidence of Murine Typhus has dropped considerably in the past few years.The monitoring result providesthe scientific basis for rodent control and disease prevention.

The authors made an investigation on fauna distribution in Huangcang Valley, XiaoCounty Huaibei Anhui Province'in Sept.1995.The authors divided this small geographically monotonousvalley into three sections as their landscape variations: conifer tree area, decidous-broadleaf tree area andcultivated & uncultivated land area.A total number of 119 rodent specimens in 7 species were caught.The rodent desity was 19.83% in the cultivated & uncultivated land-the highest among the threeareas. Apoemu agrarius, being the dominate species, 58.34% ocupation in rodent population.The rodent density in decidous-broadleaf tree area was 13.53% and 72.98% of which was ocupied by Rattusnivirenter.The lowest density was found in conifer tree area.It was only 7.53% with dominant Apodemus draco taking 61.90% ocupation.The Rattus niviwnter & Apodemus draco were the first time tohave been found in Huaibei Anhui Province.Of all the 7 species, one belongs to Oriental realm species;three are widely spread species; three are Palaearctic species.The authors consider that the fauna distribution in Huangcang Valley falls into Palaearctic realm according to total composition and dominant species,however there is obvious color of transition.

The paper reports the results of TN40-B Shashujing rice baits, whose active ingredientsare 0.005% Bromadiolone and 0.1% Trebon, against Norway rats in Dalian port, Liaoning Province,China, in May 1996.The bait palatability to Norway rats was good, the control rate reached 90 ～ 99% 11days after treatment in the field.

The survey of the host animals and chigger mite from natural focis of HFRS was carriedout from March, 1986 to February, 1987 in Qingyuan county east mountain areas of Liaoning province.1.199 rodents were caught.They belonged to eight species, seven genera.Among them, Apodemusagrarius was the predominant species which was 54.27 percent.The rate with mites was high (75%）.There appeared 3 peaks of rodent density in June, August and October to November.The highest one wasin August.2.1791 chigger mites were collected.Among them, Neotrombicula japonica was the dominantspecies, whose constituent rate was 50, 98 percent.The density of the mite was high in October.3. Neotrombicula japonica may play an important role in spreading HFRS in the fool.This providethe clue for further virology research.

In recent years, the investigations of malaria vectors in the outbreak spots were conductedby man bite collection at 166 sites of 23 counties in Guangdong Province.Seven species of Anophelineswith a total of 13236 were caught.The ratios were 48.8% (6463/13236） for An.sieaesie, 24.0%(3718/13236) for An.anthropophagus, 14.1% (1865/13236) for An.minimus and 8.1% (1070/13236) for An.candidiensis respectively.Only 5.0% (660/13236) of the ratio was others of Anophelines.Anophelines survey by cattle shed collection was conducted also.The ratios of these were 26.4%,0.3%, 42.4% and 9.5% respectively, but 21.4% Of the ratio was others of Anophelines. An.anthropophagus, the important vector of Guangdong Province, was found in eleven counties of GuangdongProvince.The positive sporozoite with four species of Anophelines had been found widely.The positive sporozoite rates were by 0.15 - 0.93% for An.siensie, 0.35 - 0.54% for An.anthropophagus,0.23 5.94% for An.minimus and 0.05% for An.candofiensis respectively.

Embryonated hen's eggs was tried to use to isolate sPOtted fever group (SFG) rickettsiaedirectly from the field samples while experimental animal was using.Two strains of SFG rickettsiae wereisolated directly from the field samples by using embryonated hen's eggs, no rickettsia was isolated by using experimental animal.The result suggested that embryonated hen's eggs couldbe used to isolate SFGrickettsiae directly from the field samples.

The studies showed that Yunnan strain of Aedes albopictus could be infected and multiply dengue (DEN) and Japanese encephalitis (JE) viruses in their bodies by ingesting blood-virus meal,or biting viremic chickens.Infected female mosquitoes were capable of transmitting DEN and JE virusesto suckling mice and chickens by biting and blood-sucking.Their transmission rates to suckling mice were22.22% for DENI, 37.50% for DEN2, 25.00% for DEN3, 44.44% for DEN4 and 37.50% for JE onthe 10th day after infection, and transmission rates to chickens were 100% for DEN4 and 66.67% forJE on the 10th day after infection.These results indicate that Ae.albopictus mosquitoes have the potential to play a role in the maintenance and transmission of DEN and JE viruses in nature.

in this paper, a 120hp kDNA fragments of Leishmania from cutaneous lesions of 2 parasitologically confirmed patients and 3 Leishmania species ( L.tropica L.infantum and L.gerbilli)were directly amplified with primer 13A and 13B.Southern hybridization were conducted with digoxigenin labelled L.tropica, L.infantum, L.gerbilli kDNA probes respectively.Results showed thatstrong hybridization was found to occur only in CL-PCR-AP with L.tropica kDNA probe, while L.infantum and L.gerbilli kDNA probes showed no detectable signals.Our results confirmed that homologous sequences exists in kDNA of L.tropica and the species causing CL in.Xinjiang, China.

Experimental rabbit model of lyme disease was duplicated in this test.23 items of bloodchemistry were dynamiclly observed.The results showed that Glutamic pyruvic transaminase, Gammaglutamyl transpeptidase, Lactate dehydrogenase, Glutamic oxalacetic transpeptidase, Hydroxybutyrate dehydrogenase, Creatine kinase, Cholesterol.Blabs urea nitrogen were risen with the development of lymedisease.Conversely, Glucose, uric acid, Phosphorus were degraded.