%0 Journal Article %A CHEN Yan-hua %A KANG Xun %A REN Xin-yue %A XIA Qian-feng %A XIE Xue-yan %A TAN Xiao-ning %T Isolation and identification of gut microbiota of Aedes albopictus larvae from Haikou and surrounding areas, China %D 2022 %R 10.11853/j.issn.1003.8280.2022.02.006 %J Chinese Journal of Vector Biology and Control %P 201-206 %V 33 %N 2 %X Objective To study the species and diversity of gut microbiota of Aedes albopictus larvae collected in Haikou and five surrounding sites, China. Methods Ae. albopictus larvae were collected in Haikou and five surrounding sites in 2019. Polymerase chain reaction amplification and product sequencing were performed for gut microbiota of the larvae. The nucleotide sequences of the larvae and gut microbiota were analyzed for homology through the National Center for Biotechnology Information database. Results A total of 8 strains of bacteria and 2 strains of fungi, belonging to 4 genera in 4 families of 2 phyla, were identified in the gut microbiota of Ae. albopictus larvae, with the dominant microflora from the Firmicutes. Bacillus cereus was isolated from the intestinal tract of the larvae in Xiyixia village and Dading village of Haikou city, and Gaodui village, Baoluo town of Wenchang city Fungi were isolated only from the larvae in Changgong village of Lingao county. Ae. albopictus larvae in Xiyixia village, Haikou had the most species of bacteria isolated from the intestinal tract, including B. velezensis, Lysinibacillus fusiformis, B. cereus, and Acinetobacter baumannii. Conclusion Gut bacteria of Ae. albopictus larvae are mainly from the Firmicutes in Haikou and five surrounding sites. The species diversity of bacteria is greater than that of fungi among gut microbiota. %U http://www.bmsw.net.cn/EN/10.11853/j.issn.1003.8280.2022.02.006