Objective To investigate the differences in feeding amount and intestinal metabolism of Blattella germanica and Periplaneta americana on 2.5% imidacloprid and 0.5% dinotefuran cockroach baits, so as to provide a theoretical basis for the difference research on lethal speed of these two effective ingredients in B. germanica and P. americana. Methods The feeding amounts of B. germanica and P. americana on baits was measured, and intestinal dissection and fecal collection were conducted. Liquid chromatography was used to analyze the content of two effective constituents in the intestinal tract and feces, and the midgut metabolic rate and digestive tract metabolic rate were calculated. The differences in the data were compared by the independent samples t test. Results The feeding amounts of B. germanica and P. americana on the two baits was significantly lower than that on the normal baits [ In B. germanica, dinotefuran: (7.56±0.79) mg/cockroach, t=-62.310, P<0.001; imidacloprid: (0.66±0.29) mg/cockroach, t=-25.873, P<0.001. In P. americana, dinotefuran: (9.53±0.58) mg/cockroach, t=-34.067, P<0.001; imidacloprid: (2.72±0.28) mg/cockroach, t=-27.191, P<0.001]. The digestive tract metabolic rate and midgut metabolic rate of 2.5% imidacloprid bait were 96.17% and 81.04% in B. germanica, and 92.71% and 97.01% in P. americana, respectively, with no significant difference between the two species (F=7.290, P=0.054; F=5.436, P=0.080). The digestive tract and midgut metabolic rates of 0.5% dinotefuran bait were 54.69% and 78.82% in B. germanica, while dinotefuran was not detected in the anterior, middle, and posterior intestines and feces of P. americana. Conclusions Both B. germanica and P. americana exhibit significant resistance to imidacloprid and dinotefuran baits, which may be one of the reasons for the difference in lethal speed of the two baits and the main reason for the poor lethal effect of dinotefuran on P. americana. Digestive tract metabolism is not the reason for the difference in lethal speed of the two baits.
TANG Li-ping, LAI Yun-yan, LIANG Ze-quan, ZHU Jian
. Metabolism of two insecticide effective ingredients in Blattella germanica and Periplaneta americana[J]. Chinese Journal of Vector Biology and Control, 2024
, 35(6)
: 648
-651
.
DOI: 10.11853/j.issn.1003.8280.2024.06.004
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