Objective To investigate the level of the anti-Japanese encephalitis virus (JEV) IgG antibody in healthy people and swine in Longnan,Gansu province,China,and to provide laboratory data for the prevention and control of Japanese encephalitis.Methods An on-site investigation was conducted from October 23 to November 15,2018.Li county,Xihe county,Wudu district,and Hui county in Longnan were chosen as monitoring points.Stratified cluster sampling was performed to select three administrative villages or communities each from the four counties or districts,and then from eight age groups (0-,2-,5-,10-,15-,30-,45-,and ≥ 60 years old),10 people each were randomly selected for investigation.From each monitoring point,pigs born in 2018 were randomly selected,and 20 porcine serum specimens were collected.Indirect enzyme-linked immunosorbent assay was used to test the anti-JEV IgG antibody in the subjects.Results A total of 888 healthy people received testing,among whom 91.10% were positive for the anti-JEV IgG antibody.There were no significant differences in the positive rate of the anti-JEV IgG antibody between subjects of different sexes and from different regions (both P>0.05).The positive rate of the antibody ranged from 86.13% to 98.00% across different age groups,and showed a downward trend with age (χ2trend=5.683,P=0.017).The positive rate showed no significant difference between subjects with immunization history and those without immunization history (χ2=6.997,P=0.008).A total of 240 pigs were tested for the anti-JEV IgG antibody,with a positive rate of 84.17%.The positive rate was significantly different between pigs of different ages (χ2=22.090, P<0.001) and between pigs raised by intensive farming and those by free-range farming (χ2=4.360,P=0.037),but not among pigs from different regions (χ2=0.893,P=0.345).Conclusion The positive rate of the anti-JEV IgG antibody was high among healthy people and pigs in Longnan.We recommend regular monitoring of the anti-JEV IgG antibody level among healthy people and pigs,improve the Japanese encephalitis surveillance system,and strengthen immunization,in order to maintain Japanese encephalitis at a low-incidence level.
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