Objective To investigate differentially expressed miRNAs between male and female Aedes albopictus and their target mRNAs,explore miRNA-mRNA regulatory relationship in male and female Ae. albopictus,and predict the potential function of key genes, and to provide evidence for clarifying the regulatory mechanisms of sex differentiation of Ae. albopictus. Methods Through small RNA library construction and mRNA sequencing analysis,differentially expressed miRNAs and mRNAs between female and male Ae. albopictus were identified. Bioinformatic tools were used to predict the target mRNAs of the differentially expressed miRNAs. The miRNA-mRNA interaction networks were constructed using the overlapping results between the predicted target mRNAs and the sex differentially expressed mRNAs. GO and KEGG enrichment analysis were performed on the target mRNAs of miRNAs to predict the possible functions of miRNAs. Results A total of 74 sex differentially expressed miRNAs were screened out,including 8 sex-specific miRNAs. A total of 470 female-specific miRNA-mRNA pairs (down-regulated miRNAs,up-regulated mRNAs) and 481 male-specific miRNA-mRNA pairs (up-regulated miRNAs,down-regulated mRNAs) were acquired. The interaction network and functional enrichment analyses determined 4 mRNAs associated with the sex differentiation and reproduction of Ae. albopictus and 17 miRNAs that directly regulated these mRNAs. Conclusion We constructed sex differentially expressed miRNA-mRNA interaction networks of Ae. albopictus,and acquired 17 miRNAs and 4 mRNAs that were related to sex differentiation, laying the foundation for further research on the role of miRNAs in regulating the sex differentiation of Ae. albopictus.
LIU Wen-juan, ZHANG Ke-xin, CHENG Peng, ZHANG Xin-yu, ZHANG Qian, ZHANG Zhong, ZHANG Rui-ling
. Deciphering sex differentially expressed miRNAs and miRNA-mRNA regulatory networks of Aedes albopictus[J]. Chinese Journal of Vector Biology and Control, 2022
, 33(2)
: 191
-200
.
DOI: 10.11853/j.issn.1003.8280.2022.02.005
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