Objective To investigate the type distribution and molecular characteristics of Yersinia enterocolitica in Anhui province, China, 2018-2019. Methods Different types of samples were collected from patients with diarrhea, animal feces, and food from 2018 to 2019, Y. enterocolitica strains were isolated to perform systematic biochemical identification, serotyping, polymerase chain reaction detection of virulence genes, and molecular typing by pulsed-field gel electrophoresis (PFGE). Results Among 2 186 samples, a total of 97 strains of Y. enterocolitica were isolated, with O:3 (21/97, 21.65%) and O:5 (17/97, 17.53%) being the main serotypes. Virulence gene analysis showed that 19.59% (19/97) of the strains were attachment invasion locus gene-positive (ail+), Yersinia stable toxin A gene-positive (ystA+), Yersinia stable toxin B gene-negative (ystB-), Yersinia adhesin A gene-positive (yadA+), and yop regulon transcriptional activator gene-positive (virF+); 63.92% (62/97) were ail-, ystA-, ystB+, yadA-, and virF-. Molecular typing by PFGE showed ≥ 95.00% similarity between the strains from patients with diarrhea and the strains from the host pigs. Conclusion In Anhui province, most Y. enterocolitica strains isolated from food, environment, and some patients with diarrhea are non-pathogenic. There are pathogenic strains in the host pigs and some patients with diarrhea. Strains isolated from pigs are closely related to human infection. Pigs are the main host of pathogenic strains of Y. enterocolitica.
YUAN Yuan, SUN Yong, CHANG Hong-wei, CHEN Xiao-long, SA Nan, GAO Da-wei, SU Bin
. Type distribution and molecular characteristics of Yersinia enterocolitica in Anhui province, China, 2018-2019[J]. Chinese Journal of Vector Biology and Control, 2021
, 32(3)
: 298
-301
.
DOI: 10.11853/j.issn.1003.8280.2021.03.008
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