Detection and genotyping of <i>Borrelia burgdorferi</i> in parasitic ticks in Qiongzhong area of Hainan province, China

ZHANG Lin, WEN Shu, HOU Xue-xia, LI Sha, YANG Xiao-na, CHEN Ting, MIAO Guang-qing, FU Xiao-ying, HAO Qin, ZHU Xiong

doi:10.11853/j.issn.1003.8280.2020.04.009

Chinese Journal of Vector Biology and Control >

2020 , Vol. 31 >Issue 4: 426 - 429

DOI: https://doi.org/10.11853/j.issn.1003.8280.2020.04.009

Original Reports

Detection and genotyping of Borrelia burgdorferi in parasitic ticks in Qiongzhong area of Hainan province, China

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1 State Key Laboratory of Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China;
2 People's Hospital of Qiongzhong Li and Miao Autonomous County;
3 People's Hospital of Sanya

Received date: 2020-02-05

Online published: 2020-08-20

Supported by

Supported by the National Science and Technology Major Project of China (No. 2017ZX10303404-006-003, 2018ZX10101002), Key R&D Project of Hainan Province (No. ZDYF2017163) and Scientific Research Project of Health and Family Planning of Hainan Province (No. 16A200051,19A200039)

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Abstract

Objective To investigate the infection status of tick vectors with Borrelia burgdorferi in Qiongzhong area of Hainan province, China, and to provide a scientific basis for the local prevention and control of Lyme disease. Methods A total of 120 parasitic ticks were collected from cattle in Qiongzhong area. Polymerase chain reaction (PCR) was performed to amplify the 16S rRNA gene of the ticks, and sequence analysis was performed to identify tick species. Nested-PCR was used to determine the infection rate of B. burgdorferi in ticks, and a homology analysis was performed based on the sequencing results to determine the genotypes of B. burgdorferi. Results All of the 120 ticks were identified as Rhipicephalus microplus, among which 44 tested positive for B. burgdorferi, with a positive rate of 36.67%. The homology analysis indicated that the R. microplus in Qiongzhong area could be infected with B. burgdorferi of various genotypes, including B. afzelii (56.82%), B. yangtze (25.00%), and B. garinii (18.18%). Conclusion The parasitic ticks in Qiongzhong area of Hainan province were infected with B. burgdorferi of various genotypes, with a high infection rate and risk of Lyme disease transmission. The local investigation and surveillance of Lyme disease in humans and hosts should be enhanced.

Key words： Tick; Borrelia burgdorferi; Bioinformatic analysis; Qiongzhong area

Cite this article

ZHANG Lin, WEN Shu, HOU Xue-xia, LI Sha, YANG Xiao-na, CHEN Ting, MIAO Guang-qing, FU Xiao-ying, HAO Qin, ZHU Xiong . Detection and genotyping of Borrelia burgdorferi in parasitic ticks in Qiongzhong area of Hainan province, China[J]. Chinese Journal of Vector Biology and Control, 2020 , 31(4) : 426 -429 . DOI: 10.11853/j.issn.1003.8280.2020.04.009

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