Objective To investigate the infection status of Wolbachia and its phylogenetic relationships among common mosquitoes at Jiangsu ports, and to lay the foundation for mosquito control strategy based on Wolbachia. Methods During June to September in 2015, the light trap and carbon dioxide trap were used in 10 areas of Jiangsu ports(Suzhou, Changzhou, Wuxi, Nanjing, Nantong, Yangzhou, Taizhou, Lianyungang, Xuzhou, and Huaian), and DNA was extracted from the trapped mosquitoes, then Wolbachia wsp gene was amplified by PCR. All wsp genes were sequenced and phylogenetic analysis followed. Results We analyzed 5 species of common mosquitoes in Jiangsu ports, the total infection rate of Wolbachia was 26.80%, and the Culex pipiens complex infection rate(47.81%) was the highest, followed by Armigeres subalbatus(46.24%), Aedes albopictus(34.06%), and Cx. tritaeniorbynchus(1.25%). Anopheles sinensis were not infected. The mosquito Wolbachia wsp gene shared 10 haplotypes, and genetic distances between these haplotypes were 0.002-0.263, Hap-1 Hap-7, Hap-8, Hap-9, and Hap-10 were unique for the same mosquito species, while Hap-2, Hap-4, and Hap-5 in Jiangsu port mosquitoes appeared at high frequency, being shared in the 2-3 species. Among them, Hap-1 and Hap-2 belong to A group, while the rest belong to B group. Conclusion The common mosquitoes of Jiangsu ports have carried 2 species of Wolbachia, its intraspecific and interspecific distribution was highly variable. Through this investigation, the infection of Wolbachia at Jiangsu ports is preliminarily understood, the survey will serve the key of biological control based on the characteristics of Wolbachia.
LIU Qian-qian, WU Bing-yao, HE Jiang, HU Shuang-shuang, YANG Qing-gui, SUN Li-xin
. Investigation on Wolbachia infection of mosquitoes in Jiangsu ports[J]. Chinese Journal of Vector Biology and Control, 2018
, 29(2)
: 138
-142
.
DOI: 10.11853/j.issn.1003.8280.2018.02.006
[1] Werren JH. Biology of Wolbachia[J]. Annu Rev Entomol,1997, 42:587-609.
[2] Bourtzis K,Dobson SL,Xi ZY,et al. Harnessing mosquito-Wolbachia symbiosis for vector and disease control[J]. Acta Trop,2014,132 Suppl:S150-163.
[3] 赵彤言,陆宝麟. 中国尖音库蚊复合组生物分类学的研究:雄蚊阳茎DV/D的数值分析[J]. 昆虫学报,1994,37(4):446-449.
[4] 张金光,霍新北. 淡色库蚊的抗性机制研究进展及控制策略[J]. 预防医学论坛,2011,17(4):355-356,358.
[5] 刘维德. 蚊类抗药性及其测定[M]. 北京:科学出版社,1979:53-63.
[6] 中华人民共和国卫生部. GB/T 26347-2010蚊虫抗药性检测方法生物测定法[S]. 北京:中国标准出版社,2011.
[7] Iturbe-Ormaetxe I,Walker T,O'Neill SL. Wolbachia and the biological control of mosquito-borne disease[J]. EMBO Rep, 2011,12(6):508-518.
[8] van den Hurk AF,Hall-Mendelins,Pyke AT,et al. Impact of Wolbachia on infection with Chikungunya and yellow fever viruses in the mosquito vector Aedes aegypti[J]. PLoS Negl Trop Dis,2012,6(11):e1892.
[9] Werren JH,Windsor DW,Guo LR,et al. Distribution of Wolbachia among neotropical arthropods[J]. Proc Roy Soc B Biol Sci, 1995,262(1364):197-204.
[10] 潘晓玲,刘起勇,奚志勇. 基于昆虫共生菌沃尔巴克氏体的蚊媒和蚊媒病控制研究进展[J]. 中国媒介生物学及控制杂志, 2014,25(1):1-7.
[11] Braig HR, Zhou WG, Dobson SL, et al. Cloning and characterization of a gene encoding the major surface protein of the bacterial endosymbiont Wolbachia pipientis[J]. J Bacteriol, 1998,180(9):2373-2378.
[12] Zhou WG,Rousset F,O'Neill S,et al. Phylogeny and PCR-based classification of Wolbachia strains using wsp gene sequences[J]. Proc Roy Soc B Biol Sci,1998,265(1395):509-515.
[13] 宋社吾,赵彤言,董言德,等. 我国蚊虫体内感染的Wolbachia的wsp基因序列测定与分析[J]. 昆虫学报,2002,45(5):571-577.
[14] 宋社吾,赵彤言,董言德,等. 我国蚊虫中昆虫共生微生物Wolbachia感染的研究[J]. 中国媒介生物学及控制杂志, 2002,13(1):19-21.
[15] Kittayapong P,Baisley KJ,Baimai V,et al. Distribution and diversity of Wolbachia infections in Southeast Asian mosquitoes (Diptera:Culicidae)[J]. J Med Entomol,2000,37(3):340-345.
[16] Ricci I,Cancrini G,Gabrielli S,et al. Searching for Wolbachia (Rickettsiales:Rickettsiaceae) in mosquitoes (Diptera:Culicidae):large polymerase chain reaction survey and new identifications[J]. J Med Entomol,2002,39(4):562-567.
[17] 赵彤言,董言德,朱礼华,等. 骚扰库蚊与尖音库蚊复合组其它亚种杂交的研究[J]. 寄生虫与医学昆虫学报,1998,5(1):41-44.
