ISSN 1003-8280 CN 10-1522/R 中国疾病预防控制中心 主办
Chinese Journal of Vector Biology and Control >
Expression profile of aminopeptidase N in different instars of Culex pipiens pallens larvae
Received date: 2016-06-16
Online published: 2016-10-20
Supported by
Supported by the National Natural Science Foundation of China (No. 81271877)
Objective To analyze the expression levels of CpAPN in different instars of Culex pipiens pallens. Methods The APN cDNA sequence was identified and sequenced by quantitative Real-time PCR (qRT-PCR), and the relative expression levels of CpAPN were assessed in different instars of larvae using qRT-PCR assays. Results The full-length of CpAPN was 1 383 bp that encoded a protein of 460 amino acid residues. The homology with Cx. pipiens quinquefasciatus (XM001862270.1) was 100%. The qRT-PCR showed that APN was expressed at all stages of Cx. pipiens pallens, with the highest level in the 4th instar and the lowest level in the 3rd instar. There was no significant difference among the 1st, 2nd and 4th instars (F=2.465,P=0.250), but they were all higher than the 3rd instar significantly (F=49.191, P=0.000), which were 3.5 times, 3.7 times, and 4.0 times of the expression levels to the 3rd instar, respectively. Conclusion CpAPN showed significantly different expression levels in different instars of Cx. pipiens pallens. The results provided experimental evidences for revealing the function of CpAPN of the mosquitoes.
Key words： Culex pipiens pallens; Aminopeptidase N; Instar; Larvae; Expression profiling
GUO Xiu-xia, YANG Pei-pei, CHENG Peng, WANG Hai-fang, LIU Li-juan, ZHANG Chong-xing, WANG Huai-wei, GONG Mao-qing . Expression profile of aminopeptidase N in different instars of Culex pipiens pallens larvae[J]. Chinese Journal of Vector Biology and Control, 2016 , 27(5) : 450 -453 . DOI: 10.11853/j.issn.1003.8280.2016.05.007
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