ISSN 1003-8280 CN 10-1522/R 中国疾病预防控制中心 主办
Chinese Journal of Vector Biology and Control >
DNA barcoding and morphological identification of Sarcophaga australis－a non-recorded flesh fly species in China
Received date: 2014-12-22
Online published: 2015-06-20
Supported by
Supported by the National Key Technology R & D Program (No. 2012BAK11B05) and Administration of Quality Supervision,Inspection and Quarantine Science and Technology Project(No. 2012IK223)
Objective To identify the flesh fly from an entry ship rapidly and accurately with‘Barcoding and Morphology’ techniques. Methods A flesh fly specimen was collected by Zhongshan CIQ on an entry ship from Australia with grapes. Morphological identification was conducted, and genomic DNA were extracted from a hind-leg of the fly. Target fragments were amplified with animal DNA barcoding universal primers (LCO1490 and HCO2198), then purified, cloned, sequenced and blasted in BOLD, a NJ tree was built based on the original data. Results From the morphological identification, it is a female Sarcophaga sp. Because of the lack of the references for female flesh flies, we could only identify it to a female Sarcophaga sp. Sequence of the DNA barcode of it was 100% identical to the published sequence of Sarcophaga australis in BOLD.‘Science and technology novelty search’result showed that it is a non-recorded species in China. Conclusion Based on the results, it could be concluded that the fly was a female S. australis. It is intercepted for the first time at China port.‘B & M’techniques is a good tool to identify the intercepted non-recorded fly species.
LIU De-xing, YUE Qiao-yun, LIAO Jun-lei, WEI Xiao-ya, CHEN Jian, HU Jia . DNA barcoding and morphological identification of Sarcophaga australis－a non-recorded flesh fly species in China[J]. Chinese Journal of Vector Biology and Control, 2015 , 26(3) : 282 -285 . DOI: 10.11853/j.issn.1003.4692.2015.03.016
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