Objective To investigate the species composition and population density of mosquitoes and the prevalence of Japanese encephalitis virus (JEV) infection in Linyi county of Shanxi province, China. Methods Mosquitoes were collected by light traps and used for identification of JEV. NS1 sequences of JEV were amplified using RT-PCR from mosquito nucleic acid. Samples with positive amplification of NS1 were further sequenced. Results A total of 4424 mosquitoes (6 species, 4 genera) were collected, of which 52.0% were Culex tritaeniorhynchus, 41.2% were Cx. pipiens pallens, and few were Anopheles sinensis and other species. Mosquito density gradually increased from June to August. The constituent ratio of Cx. tritaeniorhynchus among all mosquitoes increased month by month. A total of 2109 individuals of Cx. tritaeniorhynchus were pooled into 77 batches before nucleic acid detection of JEV, of which 10 batches were positive. Five of the positive samples were sequenced, and alignment with GenBank sequences indicated the presence of genotypeⅠ JEV. Conclusion The mosquito density is high in July and August in Linyi county, and the constituent ratio of Cx. tritaeniorhynchus and JEV positive rate are relatively high. The two indices could be used as important early warning indicators for prevention of Japanese encephalitis.
DAI Pei-fang, ZHAO Jun-ying, KONG Xiang-sheng, LIU Mei-de, ZHANG Jiu-song, LIU Zhu-ping,HAO Xiao-feng, ZHANG Hong-zi, CHENG Jing-xia
. Investigation of Japanese encephalitis vectors in 2009 in Linyi countyof Shanxi province, China[J]. Chinese Journal of Vector Biology and Control, 2014
, 25(5)
: 424
-426
.
DOI: 10.11853/j.issn.1003.4692.2014.05.010
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