Expert Forum

Phylogenetic relationship among some species of genus Anopheles subgenus Anopheles (Diptera:Culicidae) in China:based on rDNA-ITS2 sequences

Expand
  • Department of Tropical Infectious Diseases, Second Military Medical University, Shanghai 200433, China

Received date: 2013-05-20

  Online published: 2013-10-20

Supported by

Supported by the National Natural Science Foundation of China-Yunnan Joint Fund (No. U0932604)

Abstract

Objective To clarify the interspecific phylogenetic relationship among the species of genus Anopheles subgenus Anopheles in China. Methods The phylogenetic relationship among 22 species (molecular forms) of subgenus Anopheles collected from China was reconstructed based on ribosomal DNA second internal transcribed spacer (rDNA-ITS2) sequences using neighbour joining, maximum parsimony, minimum evolution and maximum likelihood methods. Results The length range of rDNA-ITS2 was 340-1754 bp; the intraspecific genetic distances (p distance) were below 0.004, and the range of pairwise genetic distances among Anopheles mosquitoes was 0.009-0.420 (mean 0.224). All the reconstructed phylogeny trees showed that there were three clades, i.e., An. hyrcanus group, An. barbirostris group and An. lindesayi with An. maculipennis group and that the group clades were paraphyletic. There were four major clusters in An. hyrcanus group, and the interspecific relationship in each cluster was the same as the morphological taxonomy. However, the cluster positions of spLL1 and spLL2 were unstable. Conclusion The molecular phylogenetic tree reconstructed in this study objectively shows the interspecific phylogenetic relationship among the groups and species of genus Anopheles subgenus Anopheles in China. The rDNA-ITS2 sequences have sufficient contribution to the reconstruction of phylogeny of Anopheles species.

Cite this article

MA Ying, MA Ya-jun, LIN Lin, WANG Yan . Phylogenetic relationship among some species of genus Anopheles subgenus Anopheles (Diptera:Culicidae) in China:based on rDNA-ITS2 sequences[J]. Chinese Journal of Vector Biology and Control, 2013 , 24(5) : 382 -388 . DOI: 10.11853/j.issn.1003.4692.2013.05.002

References


[1] 陆宝麟,许锦江,俞渊,等. 中国动物志. 昆虫纲. 第9卷. 双翅目:蚊科(下卷)
[M]. 北京:科学出版社,1997:1-62.

[2] Harbach RE, Howard TM. Index of currently recognized mosquito species (Diptera: Culicidae)
[J]. Eur Mosq Bull,2007,23:1-66.

[3] Harbach RE. Mosquito taxonomic inventory
[EB/OL].
[2012-09-28]Anopheles classification. http://mosquito-taxonomic-inventory.info/anopheles-classification.

[4] Mohanty A, Swain S, Kar SK, et al. Analysis of the phylogenetic relationship of Anopheles species, subgenus Cellia (Diptera: Culicidae) and using it to define the relationship of morphologically similar species
[J]. Infect Genet Evol,2009,9(6):1204-1224.

[5] Dixit J, Srivastava H, Sharma M, et al. Phylogenetic inference of Indian malaria vectors from multilocus DNA sequences
[J]. Infect Genet Evol,2010,10(6):755-763.

[6] Bourke BP, Foster PG, Bergo ES, et al. Phylogenetic relationships among species of Anopheles (Nyssorhynchus) (Diptera: Culicidae) based on nuclear and mitochondrial gene sequences
[J]. Acta Trop, 2010,114(2):88-96.

[7] Krzywinski J, Grushko OG, Besansky NJ. Analysis of the complete mitochondrial DNA from Anopheles funestus: an improved dipterans mitochondrial genome annotation and a temporal dimension of mosquito evolution
[J]. Mol Phylogenet Evol,2006,39(2): 417-423.

[8] Logue K, Chan ER, Phipps T, et al. Mitochondrial genome sequences reveal deep divergences among Anopheles punctulatus sibling species in Papua New Guinea
[J]. Malaria J,2013,12:64.

[9] Ambrose L, Riginos C, Cooper RD, et al. Population structure, mitochondrial polyphyly and the repeated loss of human biting ability in anopheline mosquitoes from the southwest Pacific
[J]. Mol Ecol,2012,21:4327-4343.

[10] 吴静,马雅军,马颖. 基于mtDNA和rDNA基因序列的中国按蚊属塞蚊亚属种类的系统发育研究
[J]. 昆虫学报,2011,53(9): 1030-1038.

[11] 马雅军,吴静,马颖. 基于rDNA-ITS2序列的中国按蚊属塞蚊亚属部分种类的系统发育研究
[J]. 昆虫分类学报,2011,33(4): 245-256.

[12] 马颖,马雅军,孟祥梅,等. 我国辽宁省赫坎按蚊种团的分子鉴别研究
[J]. 寄生虫与医学昆虫学报,2009,16(4):224-227.

[13] Collins FH, Mendez MA, Rasmussen MO, et al. A ribosomal RNA gene probe differentiates member species of the Anopheles gambiae complex
[J]. Am J Trop Med Hyg,1987,37(1):37-41.

[14] Porter CH, Collins FH. Species diagnostic differences in a ribosomal DNA internal transcribed spacer from the sibling species Anopheles freeborni and Anopheles hermsi (Diptera: Culicidae)
[J]. Am J Trop Med Hyg,1991,45(2):271-279.

[15] 伍桐,马雅军. rDNA-ITS2序列作为中国按蚊分子鉴别特征的评述:Ⅰ.按蚊亚属
[J]. 寄生虫与医学昆虫学报,2013,20(1): 16-24.

[16] Harbach RE. The classification of genus Anopheles (Diptera: Culicidae): a working hypothesis of phylogenetic relationships
[J]. Bull Entomol Res,2004,946:537-553.

[17] Foley DH, Bryan JH, Yeates D. Evolution and systematics of Anopheles: insights from a molecular phylogeny of Australasian mosquitoes
[J]. Mol Phylogenet Evol,1998,9(2):262-275.

[18] Sallum MAM, Schultz TR, Wilkerson RC. Phylogeny of Anophelinae (Diptera: Culicidae) based on morphological characters
[J]. Ann Entomol Soc Am,2000,93(4):745-775.

[19] Harbach RE, Kitching LJ. Reconsideration of anopheline mosquito phylogeny (Diptera: Culicidae: Anophelinae) based on morphological data
[J]. Syst Biodivers,2005,3(4):345-374.

[20] Park MH, Choochote W, Junkum A, et al. Reproductive isolations of Anopheles sinensis from Anopheles lesteri and Anopheles sineroides in Korea
[J]. Genes Genomics,2008,30(3):245-252.

[21] Ma YJ, Xu JN. The Hyrcanus group of Anopheles (Anopheles) in China (Diptera: Culicidae): species discrimination and phylogenetic relationships inferred by ribosomal DNA internal transcribed spacer 2 sequences
[J]. J Med Entomol,2005,42(4): 610-619.

[22] 陈锡欣,李石柱,邓绪礼,等. 山东省赫坎按蚊复合体成员种的分子鉴别研究
[J]. 中国寄生虫病防治杂志,2003,16(4):232-234.

[23] 林琳,杨曼尼,南春燕,等. 中国部分地区赫坎按蚊种团的分子鉴别研究
[J]. 中国媒介生物学及控制杂志,2013,24(2):93-98.

[24] 马雅军,瞿逢伊. 我国赫坎按蚊复合体成员种的rDNA-ITS2区序列差异及系统发育分析
[J]. 昆虫知识,2002,39(3):209-214.

[25] Hwang UW. Revisited ITS2 phylogeny of Anopheles (Anopheles) hyrcanus group mosquitoes: reexamination of unidentified and misidentified ITS2 sequences
[J]. Parasitol Res,2007,101: 885-894.

[26] Paredes-Esquivel C, Harbach RE,Townson H. Molecular taxonomy of members of the Anopheles hyrcanus group from Thailand and Indonesia
[J]. Med Vet Entomol,2011,25:348-352.

[27] Harrison BA, Scanlon JE. The subgenus Anopheles in Thailand (Diptera: Culicidae)
[J]. Contr Am Entomol Inst,1975,12:1-307.

[28] Paredes-Esquivel C, Donnelly MJ, Harbach RE, et al. A molecular phylogeny of mosquitoes in the Anopheles barbirostris subgroup reveals cryptic species: implications for identification of disease vectors
[J]. Mol Phylogenet Evol,2009,50(1):141-151.

[29] Suwannamit S, Baimai V, Otsuka Y, et al. Cytogenetic and molecular evidence for an additional new species within the taxon Anopheles barbirostris (Diptera: Culicidae) in Thailand
[J]. Parasit Res,2009,104(4):905-918.

[30] Saeung A, Otsuka Y, Baimai V, et al. Cytogenetic and molecular evidence for two species in the Anopheles barbirostris complex (Diptera: Culicidae) in Thailand
[J]. Parasit Res,2007,101(5): 1337-1344.

[31] Saeung A, Baimai V, Otsuka Y, et al. Molecular and cytogenetic evidence of three sibling species of the Anopheles barbirostris form A (Diptera:Culicidae) in Thailand
[J]. Parasit Res,2008,102(3): 499-507.

[32] Townson H, Dyer N, Mcakister E, et al. Systematic of Anopheles barbirostris van der Wulpand a sibling species of the Barbirostris Complex (Diptera: Culicidae) in eastern Java, Indonesia
[J]. Syst Entomol,2013,38:180-191.

[33] Otsuka Y. Variation in number and formation of repeat sequences in the rDNA ITS2 region of five sibling species in the Anopheles barbirostris complex in Thailand
[J]. J Insect Sci,2011,11:137.

Outlines

/