ISSN 1003-8280 CN 10-1522/R 中国疾病预防控制中心 主办
Chines Journal of Vector Biology and Control ›› 2009, Vol. 20 ›› Issue (6): 558-560.
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LI Yu-Chun, DU Zun-Wei, WANG Xue-Zhong, ZHANG Zai-Xing
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李雨春1,杜尊伟2,王学忠2,张再兴2
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Abstract:
【Abstract】 Objective To explore feasibility and superiority of ICEstimator online software in the calculation of median lethal concentration(LC50) and its 95% confidence interval(95%CI). Methods The LC50 values of dipterex and beta?cyfluthrin to Aedes albopictus and Culex pipiens quinquefasciatus were calculated by ICEstimator, SPSS 13.0, SAS 9.0 and DPS 7.55, respectively. Results The LC50 value of beta?cyfluthrin to Ae. albopictus and Cx. pipiens quinquefasciatus and its 95%CI, calculated by the above mentioned softwares , were 5.03(4.68-5.38), 5.20(4.90-5.60), 5.14(4.83-5.46), 5.14 (4.83-5.46), 5.31(4.58-6.03), 5.40(4.75-6.15), 5.28 (2.37-7.11), 5.28(2.37-7.11), and for dipterex, that were 92.92(83.27-102.58), 100.60(90.60-110.70), 96.00(87.88-105.33), 96.00(87.88-105.33) and 1123.02(998.89-1247.14), 1123.70(800.60-1652.40), 1111.91(725.47-1745.88), 1111.90(725.46-1745.87), respectively. However, there were no significant difference among three groups of median lethal concentration value (χ2=5.595, P=0.113) and their 95%CI by K?W?Test. Conclusion The LC50 value and its 95%CI could be got fast and conveniently by ICEstimator compared to other methods.
Key words: Median lethal concentration(LC50) value, ICEstimator
摘要:
【摘要】 目的 探索ICEstimator用来计算半数致死浓度(LC50)值及其95%可信区间(95%CI)的可行性和优越性。方法利用ICEstimator分别计算敌百虫对白纹伊蚊、致倦库蚊的LC50及其95%CI和氟氯氰菊酯对白纹伊蚊、致倦库蚊的LC50及其95%CI,并与目前常用的计算方法(SPSS、SAS、DPS)对上述数值进行分析比较。结果 经DPS/SAS/SPSS软件计算氟氯氰菊酯对白纹伊蚊及致倦库蚊的LC50(mg/ml)及其 95%CI分别为5.03(4.68~5.38)、5.20(4.90~5.60)、5.14(4.83~5.46)、5.14 (4.83~5.46)和5.31(4.58~6.03)、5.40(4.75~6.15)、5.28(2.37~7.11)、5.28(2.37~7.11);敌百虫对白纹伊蚊及致倦库蚊的LC50(mg/ml)及其 95%CI分别为92.92 (83.27~102.58)、100.60(90.60~110.70)、96.00(87.88~105.33)、96.00(87.88~105.33)和1123.02(998.89~1247.14)、1123.70(800.60~1652.40)、1111.91(725.47~1745.88)、1111.90(725.46~1745.87)。经非参数K?W?Test检验,二者计算的数值及其95%CI差异均无统计学意义(χ2=5.595, P=0.113)。结论 用ICEstimator可快速得到某杀虫剂对某种蚊虫的LC50及其95%CI,与SPSS和SAS方法比较,该计算方法计算过程简单,速度快,更加有效地利用了初始数据。
关键词: 半数致死浓度值, ICEstimator
CLC Number:
Q965.9
LI Yu-Chun, DU Zun-Wei, WANG Xue-Zhong, ZHANG Zai-Xing. Application of ICEstimator in the mosquito toxicology to calculate median lethal concentration[J]. Chines Journal of Vector Biology and Control, 2009, 20(6): 558-560.
李雨春1,杜尊伟2,王学忠2,张再兴2. ICEstimator在蚊虫毒理学半数致死浓度计算上的应用[J]. 中国媒介生物学及控制杂志, 2009, 20(6): 558-560.
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