Abstract: Objective To develop an in vitro arthropod repellent test device for the study of repellents against hard ticks. Methods The in vitro repellent devices for adult ticks and larval ticks were prepared by the wood strip method and filter paper method, respectively, and the repellent effects of the self-made repellent test device (SM device) were compared with those of the hard tick repellent test devices reported in the literature. The repellent rates of different repellent devices on hard ticks and the proportion of hard ticks in ineffective areas were subject to a difference analysis by the Kruskal Wallis H test of SPSS 18.0 software. Results In the test of wood strip repellent devices, the repellent rate of the SM device (test group) was (97.44±2.56)%, while that of the device in the literature was (87.67±6.23)%, with no statistically significant difference (F=2.105, P=0.220); the proportions of hard ticks in ineffective areas of the SM device were (4.45±2.22)% (test group) and (2.22±2.22)% (control group), which were less than (53.33±3.85)% (test group) and (60.00±3.85)% (control group) of the device in the literature, with statistically significant differences (test group:F=121.004,P<0.001; control group:F=168.825, P=0.001). In the test of filter paper repellent devices, the repellent rate of the SM device (test group) was (100.00±0.00)%, while that of the device in the literature was (95.83±4.17)%, with no statistically significant difference (F=1.000, P=0.374); the proportions of hard ticks in ineffective areas of the SM device were (0.00±0.00)% both in the test group and the control group, which were less than (41.67±7.26)% (test group) and (35.00±5.77)% (control group) of the device in the literature, with statistically significant differences (test group:F=32.895,P=0.005; control group:F=36.750, P=0.004). Conclusion The developed hard tick repellent test device has simple structure and is convenient, and leads to more credible experimental results. It provides a technical means for the research and development, effect determination, and drug screening of arthropod repellents.

Key words: Repellent test device, In vitro repellent test, Repellent screening, Hard tick

摘要： 目的 研制一种节肢动物体外驱避试验装置，用于硬蜱驱避剂的研究。方法 分别使用木条法及滤纸片法制备成蜱及幼蜱的体外驱避试验装置，并与文献报道的硬蜱驱蜱装置进行驱避效果比较，使用SPSS 18.0软件Kruskal-Wallis H检验将不同驱避装置对硬蜱的驱避率及无效区硬蜱占比进行差异性分析。结果 在木条攀爬法驱避装置试验中，自制驱避试验装置（自制装置）（试验组）驱避率为（97.44±2.56）%，文献中装置驱避率为（87.67±6.23）%，二者之间差异无统计学意义（F=2.105，P=0.220）；自制装置处于无效区硬蜱占比试验组为（4.45±2.22）%，对照组为（2.22±2.22）%，小于文献中装置［试验组（53.33±3.85）%，对照组（60.00±3.85）%］，且差异有统计学意义（试验组F=121.004，P<0.001；对照组F=168.825，P=0.001）；在滤纸片法驱避装置试验中，自制装置（试验组）驱避率为（100.00±0.00）%，文献中装置为（95.83±4.17）%，二者之间差异无统计学意义（F=1.000，P=0.374）；自制装置处于无效区的硬蜱试验组和对照组占比均为（0.00±0.00）%，小于文献中装置［试验组（41.67±7.26）%，对照组（35.00±5.77）%）］，且差异有统计学意义（试验组F=32.895，P=0.005；对照组F=36.750，P=0.004）。结论 研制的硬蜱驱避试验装置结构简单、使用方便、实验结果可信度高，为节肢动物驱避剂的研发和效果测定、药物筛选提供了一种技术手段。

关键词: 驱避试验装置, 体外驱避试验, 驱避剂筛选, 硬蜱