Abstract: Objective To understand the genetic type and characteristics of Hantavirus carried by Rattus norvegicus in Qinhuangdao port. Methods Real-time PCR and sequencing technology were applied to detect the nucleic acid of Hantavirus. And the homologous and phylogenetic analysis of positive sequence were also accomplished. Results One of the R. norvegicus showed positive result for Hantavirus. Homogeneous analysis demonstrated that the nucleic acid sequence of Hantavirus S gene was high identity with Seoul(SEO)virus from both home and abroad. Phylogenetic analysis showed that Qinhuangdao Hantavirus was clustering together with S3 sub- genotype of Seoul(SEO)virus, and was most closely related to the isolate from Liaoning province. Conclusion The genotype of hantavirus carried by R. norvegicus in Qinhuangdao is SEO virus and the sub-genotype is S3 which is consistent with the popular type of hantavirus in Hebei province.

Key words: Rattus norvegicus, Hantavirus, Real-time PCR, S gene

摘要： 目的 了解河北省秦皇岛口岸褐家鼠携带汉坦病毒情况及其基因型。方法 褐家鼠标本于2016年7月28日采自河北省秦皇岛港渤海船务有限公司货堆区和生活区，运用实时荧光PCR检测标本汉坦病毒核酸并进行核苷酸同源性和进化树分析。结果 从1只褐家鼠中检测到汉坦病毒核酸阳性，经汉坦病毒S基因序列测定和同源性分析，与国内外汉城型（SEO型）汉坦病毒相似性较高（87.3%~97.9%），与汉滩型（HTN型）汉坦病毒及其他类型汉坦病毒相似性较低（<55%）。系统进化树显示该病毒株与SEO型汉坦病毒汇聚一支，且与S3亚型在同一分支，与辽宁省褐家鼠中的病毒株亲缘关系最近。结论 河北省秦皇岛口岸褐家鼠携带的汉坦病毒SEO型为S3亚型，与河北省所流行的汉坦病毒种类一致。

关键词: 褐家鼠, 汉坦病毒, 实时荧光聚合酶链式反应, S基因