Chines Journal of Vector Biology and Control ›› 2016, Vol. 27 ›› Issue (4): 336-340.DOI: 10.11853/j.issn.1003.8280.2016.04.006

• Original reports • Previous Articles     Next Articles

An investigation on the harborage of three pathogens in small mammals in Beijing area, 2013

LYU Yan-ning1,2, LI Liang-chen3, DOU Xiang-feng1, CHEN Li-juan1, ZHANG Xiu-chun1, GUAN Zeng-zhi1, SUN Yu-lan1, LI Xin-yu1, WANG Quan-yi1   

  1. 1 Institute of Infectious Diseases and Endemic Diseases Control, Beijing Center for Disease Control and Prevention, Beijing 100013, China;
    2 School of Public Health, Capital Medical University;
    3 Xicheng District Center for Disease Control and Prevention
  • Received:2016-03-01 Online:2016-08-20 Published:2016-08-20

2013年北京地区小兽中3种病原携带状况调查

吕燕宁1,2, 李良辰3, 窦相峰1, 陈丽娟1, 张秀春1, 关增智1, 孙玉兰1, 黎新宇1, 王全意1   

  1. 1 北京市疾病预防控制中心传染病与地方病控制所, 北京 100013;
    2 首都医科大学公共卫生学院, 北京 100069;
    3 北京市西城区疾病预防控制中心, 北京 100120
  • 通讯作者: 王全意,Email:bjcdcxm@126.com
  • 作者简介:吕燕宁,女,副研究员,从事疾病控制研究,Email:lvyanning1971@163.com;李良辰,男,医师,从事疾病控制研究,Email:15210042925@163.com

Abstract:

Objective To investigate the pathogen harborage in small mammals in Beijing area. Methods Night trapping method was used to capture the small mammals in Beijing area and the organ tissue was obtained from the trapped small mammals. PCR and Real-time PCR methods were used to detect the specific gene of Orientia tsutsugamushi, Borrelia burgdorferi and Babesia in the small mammals tissue. Results In 500 samples obtained from Beijing area in 2013, the positive rate of O. tsutsugamushi was 2.40% which was distributed in 6 districts, and the highest was 21.43% in Haidian district. The positive rate of Babesia was 6.40% which was distributed in 11 districts, and the highest was 21.43% in Haidian district. Four samples were coinfected with O. tsutsugamushi and Babesia, and the coninfection rate was 0.80%. The B. burgdorferi was not detected in any of the rodents. Conclusion The O. tsutsugamushi harborage in small mammals in Beijing was not restricted in the area where the human cases were reported, and the surveillance of human cases, animal hosts and vectors should be reinforced in Beijing. The Babesia harborage was higher than O. tsutsugamushi both in distribution and infection rate, therefore the training of the fundamental medical practitioners and the surveillance and health education of the high risk group should be carried out in time.

Key words: Small mammals, Orientia tsutsugamushi, Borrelia burgdorferi, Babesia, Polymerase chain reaction

摘要:

目的 了解恙虫病、莱姆病、巴贝西虫病3类疾病病原体在北京市各区小兽中的感染及分布情况。方法 2013年4-10月,采用夹夜法捕获小兽并解剖获取其组织样本,采用普通PCR与实时荧光定量PCR方法,检测小兽组织中恙虫病东方体、伯氏疏螺旋体以及巴贝西虫的特异性目的基因。结果 2013年北京地区500份小兽样本中恙虫病东方体阳性率为2.40%,来自6个区,以海淀区最高(21.43%),其他区恙虫病东方体感染率为2.04%~5.00%;巴贝西虫阳性率为6.40%,来自11个区,以海淀区最高(21.43%),其他区巴贝西虫感染率为1.96%~16.67%;检出恙虫病东方体与巴贝西虫共同感染样本4份,复合感染率为0.80%;所有样本中均未检测到伯氏疏螺旋体。结论 北京地区小兽中恙虫病东方体的感染不仅局限于发现病例的地区,应进一步加强全市人间病例以及宿主和媒介的监测工作,巴贝西虫小兽间感染在分布范围和感染率方面均高于恙虫病东方体,应加强对基层医生的培训并及时开展高危人群的监测与健康教育。

关键词: 小兽, 恙虫病东方体, 伯氏疏螺旋体, 巴贝西虫, 聚合酶链反应

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