Chines Journal of Vector Biology and Control ›› 2019, Vol. 30 ›› Issue (5): 545-549.DOI: 10.11853/j.issn.1003.8280.2019.05.015

• Original Reports • Previous Articles     Next Articles

The determination study of Eospalax baileyi to bromadiolone and palatability of bromadiolone for E. baileyi in the eastern margin of the Qilian Mountains

TAN Yu-chen1, HAN Tian-hu2, XU Guo-cheng2, WEI Yan-ming3, CAI Zhi-yuan1, WANG Chan1, YAO Bao-hui1, GUO Huai-liang1, SU Jun-hu1   

  1. 1 College of Grassland Science, Key Laboratory of Grassland Ecosystem(Ministry of Education), Pratacultural Engineering Laboratory of Gansu Province, Sino-U. S. Centers for Grazing Land Ecosystem Sustainability, Gansu Agricultural University, Lanzhou 730070, Gansu Province, China;
    2 Garssland Technology Extension Station of Gansu Province;
    3 College of Veterinary Medicine, Gansu Agricultural University
  • Received:2019-04-21 Online:2019-10-20 Published:2019-10-20
  • Supported by:
    Supported by the Grassland Technology Extension General Station Project of Gansu Agriculture and Animal Husbandry Department (No. GCB2015-01), Longyuan Youth Innovation and Entrepreneurship Talents Project of Gansu Province (No. LQ20180916-2) and the Fuxi Talents Cultivation Project of Gansu Agricultural University (No. Gaufx-02J03)

祁连山东缘高原鼢鼠对溴敌隆的敏感性与适口性测定实验

谭宇尘1, 韩天虎2, 许国成2, 魏彦明3, 蔡志远1, 王缠1, 姚宝辉1, 郭怀亮1, 苏军虎1   

  1. 1 甘肃农业大学草业学院, 草业生态系统教育部重点实验室, 中美草地畜牧业可持续发展研究中心, 甘肃 兰州 730070;
    2 甘肃省草原技术推广总站, 甘肃 兰州 730020;
    3 甘肃农业大学动物医学院, 甘肃 兰州 730070
  • 通讯作者: 苏军虎,Email:sujh@gsau.edu.cn
  • 作者简介:谭宇尘,女,在读硕士,从事生化与分子生物学研究,Email:sasuke421339218@qq.com
  • 基金资助:
    甘肃省农牧厅草原技术推广总站项目(GCB2015-01);甘肃省陇原青年创新创业人才项目(LQ20180916-2);甘肃农业大学"伏羲杰出人才"培育项目(Gaufx-02J03)

Abstract: Objective To determine the sensitivity of Eospalax baileyi to bromadiolone (an anticoagulant rodenticide) and the palatability of bromadiolone for E. baileyi in the eastern margin of the Qilian Mountains, and to provide a scientific basis for prevention and control of E. baileyi in the field using bromadiolone as a rodenticide. Methods During July to August, 2018, non-invasive live cage trapping was used to capture E. baileyi in Tianzhu area in the eastern margin of the Qilian Mountains; the E. baileyi was then individually caged and fed in a laboratory environment. In a non-selective food intake test, 0.000 5% bromadiolone bait was continuously provided to the E. baileyi for 1, 3, 5, and 7 days, and food intake and death of the E. baileyi were recorded. Bliss calculating program in SPSS 19.0 software was used for regression analysis and fitting, and the half-lethal feeding period (LFP50) and the 99% lethal feeding period (LFP99) were calculated. The t-test for grouped data was applied to analyze the sensitivity and palatability. Results There was a decreasing trend in the food intake of E. baileyi, with no significant difference observed in the sensitivity of E. baileyi to bromadiolone between sexes (t=-1.232, P=0.329). The estimated values of LFP50 and LFP99 were 0.492 day and 11.897 days, respectively. The death peak of E. baileyi appeared on the sixth to the ninth day after intake of the poison bait. The first and second palatability of 0.000 5% bromadiolone for E. baileyi was 0.66 and 0.77, respectively, both of which showed a good food intake coefficient and a laboratory-corrected deratization rate of 100%. Conclusion Bromadiolone is highly toxic and palatable to the E. baileyi in Tianzhu area in the eastern margin of the Qilian Mountains with no resistance observed; therefore, 0.000 5% bromadiolone may be used to control E. baileyi effectively in this area.

Key words: Eospalax baileyi, Bromadiolone, Sensitivity, Palatability

摘要: 目的 测定祁连山东缘高原鼢鼠对抗凝血杀鼠剂溴敌隆的敏感性与适口性水平,为野外采用溴敌隆鼠药防制高原鼢鼠提供科学依据。方法 于2018年7-8月,在祁连山东缘天祝地区采用捕鼠笼非损伤捕获活的高原鼢鼠,实验室环境下单鼠单笼饲养,采用无选择性摄食试验,连续喂食1、3、5和7 d 0.000 5%的溴敌隆毒饵,记录高原鼢鼠取食和死亡情况,使用SPSS 19.0软件Bliss计算机程序,拟合回归分析,计算半致死喂食期(LFP50),99%致死喂食期(LFP99)。采用成组设计资料的t检验,分析其敏感性与适口性。结果 高原鼢鼠毒饵取食量有下降趋势,不同性别高原鼢鼠对溴敌隆的敏感性差异无统计学意义(t=-1.232,P=0.329),其LFP50和LFP99的估计值分别为0.492和11.897 d;高原鼢鼠取食毒饵后,死亡高峰期出现在第6~9天。0.000 5%溴敌隆对高原鼢鼠的首遇适口性为0.66,再遇适口性为0.77,均达到良好摄食系数,实验室校正灭鼠率均达100%。结论 溴敌隆对祁连山东缘天祝地区高原鼢鼠的毒效高、适口性好,且无抗性发生,0.000 5%溴敌隆可有效防治该地区的高原鼢鼠。

关键词: 高原鼢鼠, 溴敌隆, 敏感性, 适口性

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