Chinese Journal of Vector Biology and Control ›› 2021, Vol. 32 ›› Issue (6): 783-788.DOI: 10.11853/j.issn.1003.8280.2021.06.024

• Review • Previous Articles    

Progress of research on dust mite isolation and allergen detection methods

SHEN Peng1, CUI Yu-bao2   

  1. 1. Laboratory of Pathogenic Microorganism, Yancheng Center for Disease Control and Prevention, Yancheng, Jiangsu 224006, China;
    2. Department of Clinical Laboratory, the Affiliated Wuxi People's Hospital of Nanjing Medical University, Wuxi, Jiangsu 214023, China
  • Received:2021-03-13 Online:2021-12-20 Published:2021-12-15

尘螨分离及其变应原检测技术研究进展

沈鹏1, 崔玉宝2   

  1. 1. 盐城市疾病预防控制中心病原微生物与生物检验科, 江苏 盐城 224006;
    2. 南京医科大学附属无锡人民医院检验科, 江苏 无锡 214023
  • 通讯作者: 崔玉宝,E-mail:6801609@163.com
  • 作者简介:沈鹏,男,在读硕士,检验技师,主要从事病原生物学研究,E-mail:15189313167@163.com

Abstract: The diverse and widespread dust mites represent one of the most common allergens causing human allergic diseases. Two micrograms of Der p 1 per gram of dust may cause allergy and asthma, and 10 μg (500 mites per gram of dust) can be regarded as a main risk factor for acute asthma in patients with allergic constitution. Collecting indoor dust for dust mite isolation and allergen detection can be used to evaluate the number and distribution of dust mites, understand the relationship between dust mites and allergic diseases, and help control of dust mites and research on allergic diseases. Therefore, it is critical to adopt appropriate methods to collect and detect dust samples. Dust collection as well as dust mite isolation and detection methods are reviewed in this article to provide the foundation for dust mite research.

Key words: Dust mites, Allergen, Immunology, Molecular biology

摘要: 尘螨种类多,分布广,是引起人类变态反应性疾病最常见变应原的来源生物之一。每克灰尘中含有2 μg的屋尘螨变应原第一组分就可能引起过敏及哮喘,达到10 μg(每克灰尘中含有500只螨虫)就被视为过敏体质者发生急性哮喘的主要风险因素。收集室内灰尘进行尘螨分离及其变应原检测,可用于评价尘螨孳生数量、分布情况,了解尘螨与变态反应性疾病的关系,有助于尘螨防控及变态反应性疾病研究。采用合适的方法对尘样进行收集和检测十分重要。该文对灰尘收集、尘螨分离和检测方法进行综述,为尘螨相关研究奠定基础。

关键词: 尘螨, 变应原, 免疫学, 分子生物学

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