Objective To investigate the current status of resistance to commonly used insecticides in Aedes albopictus in Xingyi and Chishui cities of Guizhou province, China, to explore the killing effect of Bacillus thuringiensis subsp. israelensis on Ae. albopictus larvae in Chishui, and to provide a scientific basis for the prevention and control of Aedes vector and related infectious diseases. Methods In September 2018 and July 2019, Ae. albopictus larvae were collected from small water containers in communities in different locations of Xingyi and Chishui and were reared to obtain the F1 to F2 generations in the laboratory. The larval dipping method and the contact tube method recommended by WHO were used to investigate the insecticide resistance of Ae. albopictus larvae and adults, respectively, and determine the degree of insecticide resistance. Results The resistance ratios of Ae. albopictus larvae against deltamethrin, beta-cypermethrin, permethrin, and DDVP were 44.78, 39.18, 113.74, and 2.74 folds, respectively, in Xingyi city, and 38.38, 24.25, 26.37, and 1.64 folds, respectively, in Chishui city. In addition, the resistance ratios of Ae. albopictus larvae against propoxur and B. thuringiensis subsp. israelensis were 7.51 and 1.28 folds, respectively, in Chishui. The 24-hour mortality rates of adult Ae. albopictus mosquitoes in Xingyi against deltamethrin, beta-cypermethrin, permethrin, and fenitrothion were 27.22%, 57.89%, 46.75%, and 100%, respectively. The 24-hour mortality rates of adult mosquitoes in Chishui against deltamethrin, beta-cypermethrin, and permethrin were 53.00%, 93.33%, and 94.40%, respectively, and the mortality rate against propoxur was 99.20%. Conclusion Ae. albopictus larvae in both cities have developed moderate or above resistance to pyrethroid insecticides and are sensitive to DDVP. Ae. albopictus larvae from Chishui have low resistance to propoxur and are sensitive to B. thuringiensis subsp. israelensis. In both Xingyi and Chishui cities, Ae. albopictus adults have resistance or suspected resistance to pyrethroid insecticides and are sensitive to fenitrothion or propoxur. It is recommended to adhere to the principle of integrated vector management in both cities, timely monitor the level and changing trend of insecticide resistance of Ae. albopictus, suspend the use of high-resistance insecticides, and adopt an appropriate rotation or combination strategy, so as to delay the development of insecticide resistance.
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