目的 继续调查湖北省蚊媒和病毒种类及其分布状况。方法 2010年夏季在湖北省恩施州、神农架林区、江陵县和随州市采集蚊虫标本, 用组织培养法分离病毒, 用血清学和分子生物学方法对阳性病毒分离物进行鉴定, 利用生物信息学软件对新分离病毒进行序列同源性和系统进化分析。结果 采集到3属4种12 845只蚊虫标本, 共分离到38株阳性分离物, 经血清学和分子生物学鉴定, 32株阳性分离物为流行性乙型脑炎病毒(JEV), 5株阳性分离物为盖塔病毒, 1株为JEV和盖塔病毒感染混合株。JEV E基因序列进化分析显示新分离JEV均为基因Ⅰ型JEV。盖塔病毒NS2基因序列进化分析显示新分离病毒与中国河北省和韩国毒株同源性最高, 与俄罗斯分离株进化关系较远。结论 首次在湖北省分离到盖塔病毒, 并再次在湖北省分离到基因Ⅰ型JEV。

Objective To continue the investigation on the distribution of mosquito-borne arbovirus in some areas of Hubei province. Methods Mosquitoes were collected from Enshi state, Shennongjia forest region, Jiangling county and Suizhou city in the summer of 2010. Virus was isolated from these mosquitoes by cell culture. The virus isolates were identified by serological and molecular biological methods, and phylogenetic analysis was conducted on virus genome sequences. Results 12 845 mosquitoes were collected. 38 virus strains were isolated from mosquitoes. Through serological and molecular biological identification, 32 strains were identified as Japanese encephalitis virus (JEV), 5 were Getah virus (GETV), 1 was mixed strain of JEV and GETV. Phylogenetic analysis on E sequence of JEV showed all JEV strains belonged to genotype Ⅰ JEV, and analysis on NS2 sequence of GETV showed new strains that were homogenous with strains isolated from Hebei and South Korea, but heterogeneous with strain from Russia. Conclusion GETV was firstly isolated from Hubei province, and genotype Ⅰ JEV was re-confirmed from Hubei province.