目的 探索阿拉善黄鼠和达乌尔黄鼠的分类鉴定方法。方法 运用DNA条形码技术测定两地黄鼠的线粒体细胞色素C氧化酶亚基Ⅰ(COⅠ)基因序列,分析遗传距离,构建系统发育NJ树。结果 11份阿拉善黄鼠的种内遗传距离＜2%,4份达乌尔黄鼠的种内遗传距离＜1%,两地黄鼠的遗传距离介于8%～9%之间,同时,NJ树将两地黄鼠分成2个独立分支。结论 阿拉善黄鼠为独立物种。DNA条形码技术可用于黄鼠种类鉴定。
Objective To investigate the classification and identification methods for Spermophilus alaschanicus and S. dauricus. Methods DNA barcoding was used to determine the cytochrome c oxidase subunitⅠ (COⅠ) gene sequences of S. alaschanicus and S. dauricus, analyze the genetic distance, and construct a neighbor-joining (NJ) tree. Results The intraspecific genetic distance of S. alaschanicus (n＝11) was less than 2%, and that of S. dauricus (n＝4) was less than 1%. The genetic distance between S. alaschanicus and S. dauricus ranged from 8% to 9%. Results of NJ tree showed that all the samples of the two species formed two independent groups with a high support value. Conclusion Spermophilus alaschanicus is an independent species. DNA barcoding can be used in the species identification of Spermophilus.
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