中国媒介生物学及控制杂志 ›› 2009, Vol. 20 ›› Issue (2): 138-139.

• 论著 • 上一篇    下一篇

3种吸血蚋酯酶同工酶的比较研究

高博1,张建庆1,蔡亨忠1,王光辉1,范建华1,黄健群1,黄恩炯1,王宇平1,张述铿1,王公东2   

  1. 1 福建出入境检验检疫局国际旅行卫生保健中心(福州 350001); 2 福建出入境检验检疫局武夷山办事处
  • 收稿日期:2008-11-03 出版日期:2009-04-20 发布日期:2009-04-20
  • 作者简介:高博(1972-), 男,主管医师,从事医学媒介生物防控工作。

Comparison of esterase isozymes in three species of backflies (Diptera:Simuliidae)

GAO Bo, ZHANG Jian-Qing, CAI Heng-Zhong, WANG Guang-Hui, FAN Jian-Hua, HUANG Jian-Qun, HUANG En-Jiong, WANG Yu-Ping, ZHANG Shu-Keng, WANG Gong-Dong   

  1. International Travel Health Care Center of Fujian Entry?Exit Inspection & Quarantine Bureau, Fuzhou, Fujian 350001, China
  • Received:2008-11-03 Online:2009-04-20 Published:2009-04-20

摘要:

  【摘要】 目的 探讨酯酶(EST)同工酶酶谱特征在蚋类分类上的价值,并为蚋类系统发育的研究积累生物化学方面的基础资料。方法 应用垂直板不连续聚丙烯酰胺凝胶电泳技术,对素木蚋、双齿蚋和五条蚋进行EST同工酶电泳,测量并计算各酶带的迁移率。 结果 3蚋种的EST同工酶酶谱共显示10条酶带,种间存在明显差异,具有各自的特征性酶谱,种内个体之间、幼虫与蛹之间酶谱主带相同,非主带略有差异。 结论 EST同工酶酶谱可作为蚋种鉴定的特征之一。

关键词: 素木蚋, 双齿蚋, 五条蚋, 电泳, 酯酶同工酶

Abstract:

  【Abstract】 Objective  To explore the value of isozyme zymograms in the classification of blackflies and accumulate basic biochemical data for the study of phylogenesis. Methods EST isozymes of three blackfly species were studied by discontinuous PAGE techniques. Results Ten isozyme bands could be identified according to the electrophoresis zymograms. The differences of zymograms were significant between three blackfly species and each species had its own special zymogram. The main band among intraspecific individuals or between the larvae and pupae was alike, however, other enzyme bands were different. Conclusion EST isozyme zymograms could be used as one of the classification characteristics of blackflies.

Key words: Simulium (Simulium) shirakii, Simulium (S.) bidentatum, Simulium (S.) quinquestriatum, Electrophoresis, Esterase isozyme

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