中国媒介生物学及控制杂志

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中国鼠疫菌某些生化特征及流行病学意义

董兴齐1;李敏2;夏连续3;王鹏1;崔百忠2;孙连芝3;俞东征4;海荣4   

  1. 1云南省地方病防治所 大理671000;2青海省疾病预防控制所;3内蒙古自治区地方病防治研究中心;4中国疾病预防控制中心传染病预防控制所
  • 出版日期:2003-12-20 发布日期:2003-12-20

Epidemiological Significance on Some Biochemical Characters of Yersinia pestis Isolated from China

DONG Xing-qi*; LI Min; XIA Lian-xu; et al   

  1. Yunnan Institute of Endemic Diseases Control and Prevention, Dali 671000, China
  • Online:2003-12-20 Published:2003-12-20

摘要: 目的探讨中国鼠疫菌株生化表征的遗传变异与分型地位。方法采用平皿-单菌落法,实验观察我国17个生态型共336株鼠疫菌酵解麦芽糖、鼠李糖、阿胶糖、甘油及脱氮反应的变化。结果发现松辽平原、滇闽居民区和滇西纵谷区3个生态型部分菌株对甘油、阿胶糖、麦芽糖酵解有变异,可同时出现酵解和不酵解的单菌落,而且特性较稳定;其余14个生态型鼠疫菌单菌落酵解能力与过去资料报道一致无明显变化;根据实验结果可将中国鼠疫菌分为12个生化型。结论我国鼠疫菌对糖酵解特性稳定,具有一定的分型意义,滇闽居民区与滇西纵谷区生态型鼠疫菌株可能源于一个祖先。

关键词: 鼠疫菌, 生化特征, 流行病学意义

Abstract: Objective To explore the genetic variation and typing significance of biochemical characters ofYersinia pestis isolated from P.R.China.Methods To observe the change of 336 strains of.17 ecotypes with the single colony of every strain for ferments L-maltose,L-Rhamnose,L-Arabinose,and Glycerol and reduces KNO 3 by plate method.Results There were 3 ecotypes,the Songliao Plain,the Dianmian Residential Area and the Dianxi Vertical Vale with different colonies in fermenting Glycerol,Arabinose and maltose,respectively.The remainder was steady and consistent with the past experiments.17 ecotypes of Yersinia pestis could be divided into 12 biochemical types according to the study results.Conclusion Yersinia pestis strains are steady to ferment the above carbohydrates,which have a certain sense in Yersinia pestis typing.The Dianmian Residential Area and the Dianxi Vertical Vale are likely to derive from the same ancestor.