中国媒介生物学及控制杂志

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RT-PCR检测病媒生物SARS冠状病毒结果初报

段金花;吴军;林立丰;裴福全;易建荣;卢文成;蔡松武   

  1. 广东省疾病预防控制中心消杀所 广州510300
  • 出版日期:2003-10-20 发布日期:2003-10-20

Preliminary Report on SARS Coronavirus Detection from Vector Rat and Cockroach by RT-PCR

DUAN Jin-hua; WU jun; LIN Li-feng; et al   

  1. Center for Diseases Control and Prevention of Guangdong province, Guangzhou 510300,China
  • Online:2003-10-20 Published:2003-10-20

摘要: 目的调查广东重点地区鼠类和蜚蠊是否携带SARS冠状病毒或者是否SARS冠状病毒的宿主,为寻找SARS冠状病毒的来源及控制SARS流行提供依据。方法巢式RT-PCR技术、荧光定量RT-PCR技术。结果利用SARS冠状病毒特异性引物对广州、中山、江门及恩平等地的160份鼠肺样品及15份蜚蠊的体表擦拭子检测结果表明,荧光定量RT-PCR未检测到阳性,巢式RT-PCR显示来自广州市的1份蜚蠊体表擦拭子呈可疑阳性。结论巢式RT-PCR、荧光定量RT-PCR都适合于病媒生物SARS冠状病毒的初步筛选,目前未有明显证据显示鼠类及蜚蠊携带和传播SARS冠状病毒,仍需作进一步研究。

关键词: SARS冠状病毒, 巢式逆转录-聚合酶链反应, 荧光定量RT-PCR, 病媒生物

Abstract: Objective To investigate SARS coronavirus carried by or reserved in rat and cockroach,and try to provide the evidence for the origin and control of SARS coronavirus.Methods Nested reverse-transcript PCR and fluorescent quantitative reverse-transcript PCR were applied.Results With SARS Cov.-specific primer,a total of 160 rat lung tissues and 15 cockroach surface swabs were tested in nested RT-PCR and fluorescent quantitative RT-PCR.No positive was found by fluorescent quantitative RT-PCR,and only one uncertain positive result of cockroach surface by nested RT-PCR.Conclusion Nested RT-PCR and fluorescent quantitative RT-PCR are available in fast testing positive vector,and there was no evidence to regard the role of rat and cockroach in the origin and transmission of SARS coronavirus,which deserves to be studied further in the future.