中国媒介生物学及控制杂志

• 论著 • 上一篇    下一篇

昆虫生长调节剂影响德国小蠊发育繁殖的组织病理学变化及生化基础

郝蕙玲;林永丽;孙锦程;曹敏   

  1. 海军医学研究所 上海200433
  • 出版日期:2003-02-20 发布日期:2003-02-20

Pathoanatomy and Biochemical Studies on IGRs in Relation to Growth and Reproduction in Bllattella germanica

HAO Hui-ling;LIN Yong-li;SUN Jin-cheng;et al   

  1. Naval Medical Institute,Shanghai 200433,China
  • Online:2003-02-20 Published:2003-02-20

摘要: 目的探讨昆虫生长调节剂对德国小蠊生长繁殖的影响及抗几丁质合成抑制剂不育性的效果。方法采用药膜法和药饵法处理试虫,在光学显微镜和双目解剖镜下解剖观测咽侧体、脂肪体及生殖系统的病理学变化,测定受试成虫卵巢蛋白质含量及精巢、卵巢核酸含量的变化。结果抗几丁质合成抑制剂具有保幼激素的生理效应,并在德国小蠊体内有协同作用;脂肪体也是昆虫生长调节剂作用的靶器官之一;处理组卵巢蛋白质含量、卵巢RNA含量及精巢DNA及RNA含量均增加,卵巢中DNA含量减少。结论抗几丁质合成抑制剂对德国小蠊雄虫不育性的效果可能与几丁质合成受阻有关,雌性成虫不育性与卵巢中DNA合成受阻有关。

关键词: 昆虫生长调节剂, 德国小蠊, 组织病理学变化, 蛋白质, 核酸

Abstract: Objective In order to investigate the functions of IGRs to growth and reproduction of Bllattella germanica,the sterilization of chitin synthesis inhibitors against Bllattella germanica was studied too.Methods Contacting and bait methods were used and the pathology changing of CA,fat body and reproductive system were observed under stereomicroscope and microscope.Contents of the protein in ovary,DNA and RNA in ovary and spermary were determined.Results Chitin synthesis inhibitors have some juvenile hormone effect.The two are unite efforts in Bllattella germanica.Fat body is one of the target to IGRs,the contents of DNA and RNA in spermary,RNA and protein in ovary increased all but the content of DNA in ovary decreased.Conclusion The sterilization of the Chitin synthesis inhibitors in male Bllattella germanica may be related to the chitin blocking but not to the hindering of RNA and DNA,the sterilization in female Bllattella germanica may be related to the decreasing of DNA in ovary.